2qqv: Difference between revisions

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{{Seed}}
[[Image:2qqv.png|left|200px]]


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==Crystal structure of a cell-wall invertase (E203A) from Arabidopsis thaliana in complex with sucrose==
The line below this paragraph, containing "STRUCTURE_2qqv", creates the "Structure Box" on the page.
<StructureSection load='2qqv' size='340' side='right'caption='[[2qqv]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2qqv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QQV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QQV FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
{{STRUCTURE_2qqv|  PDB=2qqv  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qqv OCA], [https://pdbe.org/2qqv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qqv RCSB], [https://www.ebi.ac.uk/pdbsum/2qqv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qqv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/INV1_ARATH INV1_ARATH] Beta-fructofuranosidase that can use sucrose and 1-kestose, and, to a lower extent, neokestose and levan, as substrates, but not inuline.<ref>PMID:17963237</ref> <ref>PMID:17873089</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qq/2qqv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qqv ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In plants, cell-wall invertases fulfil important roles in carbohydrate partitioning, growth, development and crop yield. In this study, we report on different X-ray crystal structures of Arabidopsis thaliana cell-wall invertase 1 (AtcwINV1) mutants with sucrose. These structures reveal a detailed view of sucrose binding in the active site of the wild-type AtcwINV1. Compared to related enzyme-sucrose complexes, important differences in the orientation of the glucose subunit could be observed. The structure of the E203Q AtcwINV1 mutant showed a complete new binding modus, whereas the D23A, E203A and D239A structures most likely represent the productive binding modus. Together with a hydrophobic zone formed by the conserved W20, W47 and W82, the residues N22, D23, R148, E203, D149 and D239 are necessary to create the ideal sucrose-binding pocket. D239 can interact directly with the glucose moiety of sucrose, whereas K242 has an indirect role in substrate stabilization. Most probably, K242 keeps D239 in a favourable position upon substrate binding. Unravelling the exact position of sucrose in plant cell-wall invertases is a necessary step towards the rational design of superior invertases to further increase crop yield and biomass production.


===Crystal structure of a cell-wall invertase (E203A) from Arabidopsis thaliana in complex with sucrose===
Crystal structures of Arabidopsis thaliana cell-wall invertase mutants in complex with sucrose.,Lammens W, Le Roy K, Van Laere A, Rabijns A, Van den Ende W J Mol Biol. 2008 Mar 21;377(2):378-85. Epub 2008 Jan 5. PMID:18258263<ref>PMID:18258263</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2qqv" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_18258263}}, adds the Publication Abstract to the page
*[[Invertase|Invertase]]
(as it appears on PubMed at http://www.pubmed.gov), where 18258263 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_18258263}}
__TOC__
 
</StructureSection>
==About this Structure==
2QQV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QQV OCA].
 
==Reference==
Crystal structures of Arabidopsis thaliana cell-wall invertase mutants in complex with sucrose., Lammens W, Le Roy K, Van Laere A, Rabijns A, Van den Ende W, J Mol Biol. 2008 Mar 21;377(2):378-85. Epub 2008 Jan 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18258263 18258263]
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Beta-fructofuranosidase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Lammens W]]
[[Category: Ende, W Van den.]]
[[Category: Le Roy K]]
[[Category: Laere, A Van.]]
[[Category: Rabijns A]]
[[Category: Lammens, W.]]
[[Category: Van Laere A]]
[[Category: Rabijns, A.]]
[[Category: Van den Ende W]]
[[Category: Roy, K Le.]]
[[Category: Glycosidase]]
[[Category: Hydrolase]]
[[Category: Invertase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:39:18 2008''

Latest revision as of 08:34, 30 October 2024

Crystal structure of a cell-wall invertase (E203A) from Arabidopsis thaliana in complex with sucrose

2qqv, resolution 3.01Å

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