2v6e: Difference between revisions

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{{Seed}}
[[Image:2v6e.png|left|200px]]


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==protelomerase TelK complexed with substrate DNA==
The line below this paragraph, containing "STRUCTURE_2v6e", creates the "Structure Box" on the page.
<StructureSection load='2v6e' size='340' side='right'caption='[[2v6e]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2v6e]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_N15 Escherichia virus N15] and [https://en.wikipedia.org/wiki/Klebsiella_phage_phiKO2 Klebsiella phage phiKO2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V6E FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
{{STRUCTURE_2v6e|  PDB=2v6e  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6e OCA], [https://pdbe.org/2v6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v6e RCSB], [https://www.ebi.ac.uk/pdbsum/2v6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v6e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PTELO_BPKO2 PTELO_BPKO2] Converts the circular intermediates produced by the viral replication and carrying a joined telomere site to a linear DNA molecule with covalently closed hairpin ends (PubMed:14996813). The viral circular DNA is cleaved at a palindromic site called telRL thereby generating a linear prophage plasmid with telomeres (PubMed:14996813). Binds covalently to the 3'-phosphoryl of the cleaved strands (By similarity).[UniProtKB:Q77WP1]<ref>PMID:14996813</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The termini of linear chromosomes are protected by specialized DNA structures known as telomeres that also facilitate the complete replication of DNA ends. The simplest type of telomere is a covalently closed DNA hairpin structure found in linear chromosomes of prokaryotes and viruses. Bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (protelomerase). Here we report a crystal structure of the protelomerase TelK from Klebsiella oxytoca phage varphiKO2, in complex with the palindromic target DNA. The structure shows the TelK dimer destabilizes base pairing interactions to promote the refolding of cleaved DNA ends into two hairpin ends. We propose that the hairpinning reaction is made effectively irreversible by a unique protein-induced distortion of the DNA substrate that prevents religation of the cleaved DNA substrate.


===PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA===
An interlocked dimer of the protelomerase TelK distorts DNA structure for the formation of hairpin telomeres.,Aihara H, Huang WM, Ellenberger T Mol Cell. 2007 Sep 21;27(6):901-13. PMID:17889664<ref>PMID:17889664</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 17889664 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_17889664}}
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</StructureSection>
==About this Structure==
[[Category: Escherichia virus N15]]
2V6E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_phage_phiko2 Klebsiella phage phiko2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6E OCA].
[[Category: Klebsiella phage phiKO2]]
 
[[Category: Large Structures]]
==Reference==
[[Category: Aihara H]]
An interlocked dimer of the protelomerase TelK distorts DNA structure for the formation of hairpin telomeres., Aihara H, Huang WM, Ellenberger T, Mol Cell. 2007 Sep 21;27(6):901-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17889664 17889664]
[[Category: Ellenberger T]]
[[Category: Klebsiella phage phiko2]]
[[Category: Huang WM]]
[[Category: Single protein]]
[[Category: Aihara, H.]]
[[Category: Ellenberger, T.]]
[[Category: Huang, W M.]]
[[Category: Dna distortion]]
[[Category: Hairpin telomere]]
[[Category: Hydrolase]]
[[Category: Protelomerase]]
[[Category: Resolvase]]
 
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