1ngw: Difference between revisions

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{{Seed}}
[[Image:1ngw.png|left|200px]]


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==Chimeric Affinity Matured Fab 7g12 complexed with mesoporphyrin==
The line below this paragraph, containing "STRUCTURE_1ngw", creates the "Structure Box" on the page.
<StructureSection load='1ngw' size='340' side='right'caption='[[1ngw]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1ngw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NGW FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MMP:N-METHYLMESOPORPHYRIN'>MMP</scene></td></tr>
{{STRUCTURE_1ngw|  PDB=1ngw  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ngw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngw OCA], [https://pdbe.org/1ngw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ngw RCSB], [https://www.ebi.ac.uk/pdbsum/1ngw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HVM49_MOUSE HVM49_MOUSE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ng/1ngw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ngw ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The crystal structures of the Fab fragment of the germ-line precursor antibody to 7G12 and its complex with the hapten N-methylmesoporphyrin have also been solved. A comparison of these structures with the corresponding structures of the affinity-matured antibody 7G12 reveals the molecular mechanism by which the immune system evolves binding energy to catalyze this reaction.


===Chimeric Affinity Matured Fab 7g12 complexed with mesoporphyrin===
Structural evidence for substrate strain in antibody catalysis.,Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:12552112<ref>PMID:12552112</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1ngw" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_12552112}}, adds the Publication Abstract to the page
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 12552112 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_12552112}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Homo sapiens]]
1NGW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus,_homo_sapiens Mus musculus, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGW OCA].
[[Category: Large Structures]]
 
[[Category: Mus musculus]]
==Reference==
[[Category: Andryski SE]]
Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12552112 12552112]
[[Category: Beuscher IV AE]]
[[Category: Mus musculus, homo sapiens]]
[[Category: Schultz PG]]
[[Category: Single protein]]
[[Category: Stevens RC]]
[[Category: Andryski, S E.]]
[[Category: Yin J]]
[[Category: IV, A E.Beuscher.]]
[[Category: Schultz, P G.]]
[[Category: Stevens, R C.]]
[[Category: Yin, J.]]
[[Category: Antibody]]
[[Category: Antigen binding fragment]]
[[Category: Immunoglobulin]]
 
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Retrieved from "https://proteopedia.org/1ngw"