1lqj: Difference between revisions

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New page: left|200px<br /><applet load="1lqj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lqj, resolution 3.35Å" /> '''ESCHERICHIA COLI URA...
 
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[[Image:1lqj.jpg|left|200px]]<br /><applet load="1lqj" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1lqj, resolution 3.35&Aring;" />
'''ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE'''<br />


==Overview==
==ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE==
The structures of a new crystal form of free Escherichia coli uracil DNA, glycosylase (UDG), containing four molecules in the asymmetric unit, and, two forms of its complex with the proteinaceous inhibitor Ugi, containing, two and four crystallographically independent complexes, have been, determined. A comparison of these structures and the already known crystal, structures containing UDG shows that the enzyme can be considered to be, made up of two independently moving structural entities or domains. A, detailed study of free and DNA-bound human enzyme strengthens this, conclusion. The domains close upon binding to uracil-containing DNA, whereas they do not appear to do so upon binding to Ugi. The comparative, study also shows that the mobility of the molecule involves the rigid-body, movement of the domains superposed on flexibility within domains.
<StructureSection load='1lqj' size='340' side='right'caption='[[1lqj]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1lqj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LQJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqj OCA], [https://pdbe.org/1lqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lqj RCSB], [https://www.ebi.ac.uk/pdbsum/1lqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/UNG_ECOLI UNG_ECOLI] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.[HAMAP-Rule:MF_00148]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lq/1lqj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lqj ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1LQJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LQJ OCA].
*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Domain closure and action of uracil DNA glycosylase (UDG): structures of new crystal forms containing the Escherichia coli enzyme and a comparative study of the known structures involving UDG., Saikrishnan K, Bidya Sagar M, Ravishankar R, Roy S, Purnapatre K, Handa P, Varshney U, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1269-76. Epub 2002, Jul 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12136137 12136137]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Purnapatre, K.]]
[[Category: Purnapatre K]]
[[Category: Ravishankar, R.]]
[[Category: Ravishankar R]]
[[Category: Roy, S.]]
[[Category: Roy S]]
[[Category: Sagar, M.B.]]
[[Category: Sagar MB]]
[[Category: Saikrishnan, K.]]
[[Category: Saikrishnan K]]
[[Category: Varshney, U.]]
[[Category: Varshney U]]
[[Category: Vijayan, M.]]
[[Category: Vijayan M]]
[[Category: base excision]]
[[Category: dna repair]]
[[Category: glycosylase]]
[[Category: hydrolase]]
 
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Latest revision as of 07:35, 14 February 2024

ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE

1lqj, resolution 3.35Å

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