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New page: left|200px<br /><applet load="1lri" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lri, resolution 1.45Å" /> '''BETA-CRYPTOGEIN-CHOL...
 
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[[Image:1lri.gif|left|200px]]<br /><applet load="1lri" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1lri, resolution 1.45&Aring;" />
'''BETA-CRYPTOGEIN-CHOLESTEROL COMPLEX'''<br />


==Overview==
==BETA-CRYPTOGEIN-CHOLESTEROL COMPLEX==
Cryptogein is a small 10 kDa elicitor produced by the phytoparasitic, oomycete Phytophthora cryptogea. The protein also displays a sterol, carrier activity. The native protein crystallizes in space group P4(1)22, with unit-cell parameters a = b = 46.51, c = 134.9 A (diffraction limit:, 2.1 A). Its complex with cholesterol crystallizes in space group C222(1), with unit-cell parameters a = 30.96, b = 94.8, c = 65.3 A and a resolution, enhanced to 1.45 A. The large inner non-specific hydrophobic cavity is, able to accommodate a large variety of 3-beta-hydroxy sterols. Cryptogein, probably acts as a sterol shuttle helping the pathogen to grow and, complete its life cycle.
<StructureSection load='1lri' size='340' side='right'caption='[[1lri]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1lri]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phytophthora_cryptogea Phytophthora cryptogea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LRI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LRI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lri OCA], [https://pdbe.org/1lri PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lri RCSB], [https://www.ebi.ac.uk/pdbsum/1lri PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lri ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ELIB_PHYCR ELIB_PHYCR] Induces local and distal defense responses (incompatible hypersensitive reaction) in plants from the solanaceae and cruciferae families. Elicits leaf necrosis and causes the accumulation of pathogenesis-related proteins. Might interact with the lipidic molecules of the plasma membrane.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lr/1lri_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lri ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cryptogein is a small 10 kDa elicitor produced by the phytoparasitic oomycete Phytophthora cryptogea. The protein also displays a sterol carrier activity. The native protein crystallizes in space group P4(1)22, with unit-cell parameters a = b = 46.51, c = 134.9 A (diffraction limit: 2.1 A). Its complex with cholesterol crystallizes in space group C222(1), with unit-cell parameters a = 30.96, b = 94.8, c = 65.3 A and a resolution enhanced to 1.45 A. The large inner non-specific hydrophobic cavity is able to accommodate a large variety of 3-beta-hydroxy sterols. Cryptogein probably acts as a sterol shuttle helping the pathogen to grow and complete its life cycle.


==About this Structure==
The 1.45 A resolution structure of the cryptogein-cholesterol complex: a close-up view of a sterol carrier protein (SCP) active site.,Lascombe MB, Ponchet M, Venard P, Milat ML, Blein JP, Prange T Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1442-7. Epub 2002, Aug 23. PMID:12198300<ref>PMID:12198300</ref>
1LRI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytophthora_cryptogea Phytophthora cryptogea] with CL and CLR as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LRI OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The 1.45 A resolution structure of the cryptogein-cholesterol complex: a close-up view of a sterol carrier protein (SCP) active site., Lascombe MB, Ponchet M, Venard P, Milat ML, Blein JP, Prange T, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1442-7. Epub 2002, Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12198300 12198300]
</div>
<div class="pdbe-citations 1lri" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Phytophthora cryptogea]]
[[Category: Phytophthora cryptogea]]
[[Category: Single protein]]
[[Category: Blein J-P]]
[[Category: Blein, J.P.]]
[[Category: Lascombe M-B]]
[[Category: Lascombe, M.B.]]
[[Category: Milat M-L]]
[[Category: Milat, M.L.]]
[[Category: Ponchet M]]
[[Category: Ponchet, M.]]
[[Category: Prange T]]
[[Category: Prange, T.]]
[[Category: Venard P]]
[[Category: Venard, P.]]
[[Category: CL]]
[[Category: CLR]]
[[Category: cholesterol]]
[[Category: cryptogein]]
[[Category: sterol carrier protein]]
 
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Latest revision as of 06:58, 30 October 2024

BETA-CRYPTOGEIN-CHOLESTEROL COMPLEX

1lri, resolution 1.45Å

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