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New page: left|200px<br /><applet load="1lsp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lsp, resolution 2.45Å" /> '''THE CRYSTAL STRUCTUR...
 
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[[Image:1lsp.jpg|left|200px]]<br /><applet load="1lsp" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1lsp, resolution 2.45&Aring;" />
'''THE CRYSTAL STRUCTURE OF A BULGECIN-INHIBITED G-TYPE LYSOZYME FROM THE EGG-WHITE OF THE AUSTRALIAN BLACK SWAN. A COMPARISON OF THE BINDING OF BULGECIN TO THREE MURAMIDASES'''<br />


==Overview==
==THE CRYSTAL STRUCTURE OF A BULGECIN-INHIBITED G-TYPE LYSOZYME FROM THE EGG-WHITE OF THE AUSTRALIAN BLACK SWAN. A COMPARISON OF THE BINDING OF BULGECIN TO THREE MURAMIDASES==
Bulgecin A, a bacterial metabolite, has been shown to bind in the, active-site groove of the chicken-type lysozyme from the rainbow trout, (RBTL) and in the lysozyme-like C-terminal domain, of a soluble lytic, transglycosylase (C-SLT) from Escherichia coli. These enzymes are, muramidases that cleave the glycosidic bonds in the glycan strands of the, murein polymer. Here we report the crystal structure of a complex between, the goose-type lysozyme from the egg white of the Australian black swan, (SEWL) and bulgecin A at 2.45 A resolution. As is the case for the, C-SLT/bulgecin and RBTL/bulgecin complexes, the ligand binds with the, N-acetylglucosamine ring in subsite C and the proline moiety in site D, where it interacts with the catalytic glutamic acid. The taurine residue, interacts with the beta-sheet region. Comparisons of the three buigecin, complexes show that the inhibitor has the same binding mode to the, muramidases with similar protein-ligand interactions, particularly for, SEWL and RBTL. From our results, it seems likely that bulgecin, in, general, inhibits enzymes with lysozyme-like domains and thus might, represent a novel class of natural antibiotics that act on, murein-degrading rather than murein-synthesizing enzymes.
<StructureSection load='1lsp' size='340' side='right'caption='[[1lsp]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1lsp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cygnus_atratus Cygnus atratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LSP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BUL:BULGECIN+A'>BUL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lsp OCA], [https://pdbe.org/1lsp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lsp RCSB], [https://www.ebi.ac.uk/pdbsum/1lsp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lsp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LYG_CYGAT LYG_CYGAT]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ls/1lsp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lsp ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bulgecin A, a bacterial metabolite, has been shown to bind in the active-site groove of the chicken-type lysozyme from the rainbow trout (RBTL) and in the lysozyme-like C-terminal domain, of a soluble lytic transglycosylase (C-SLT) from Escherichia coli. These enzymes are muramidases that cleave the glycosidic bonds in the glycan strands of the murein polymer. Here we report the crystal structure of a complex between the goose-type lysozyme from the egg white of the Australian black swan (SEWL) and bulgecin A at 2.45 A resolution. As is the case for the C-SLT/bulgecin and RBTL/bulgecin complexes, the ligand binds with the N-acetylglucosamine ring in subsite C and the proline moiety in site D where it interacts with the catalytic glutamic acid. The taurine residue interacts with the beta-sheet region. Comparisons of the three buigecin complexes show that the inhibitor has the same binding mode to the muramidases with similar protein-ligand interactions, particularly for SEWL and RBTL. From our results, it seems likely that bulgecin, in general, inhibits enzymes with lysozyme-like domains and thus might represent a novel class of natural antibiotics that act on murein-degrading rather than murein-synthesizing enzymes.


==About this Structure==
Structure of a bulgecin-inhibited g-type lysozyme from the egg white of the Australian black swan. A comparison of the binding of bulgecin to three muramidases.,Karlsen S, Hough E, Rao ZH, Isaacs NW Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):105-14. PMID:15299731<ref>PMID:15299731</ref>
1LSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cygnus_atratus Cygnus atratus] with BUL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LSP OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of a bulgecin-inhibited g-type lysozyme from the egg white of the Australian black swan. A comparison of the binding of bulgecin to three muramidases., Karlsen S, Hough E, Rao ZH, Isaacs NW, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):105-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299731 15299731]
</div>
<div class="pdbe-citations 1lsp" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cygnus atratus]]
[[Category: Cygnus atratus]]
[[Category: Lysozyme]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Hough E]]
[[Category: Hough, E.]]
[[Category: Isaacs NW]]
[[Category: Isaacs, N.W.]]
[[Category: Karlsen S]]
[[Category: Karlsen, S.]]
[[Category: Rao ZH]]
[[Category: Rao, Z.H.]]
[[Category: BUL]]
[[Category: hydrolase (o-glycosyl)]]
 
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Retrieved from "https://proteopedia.org/1lsp"