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New page: left|200px<br /><applet load="1m1f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m1f, resolution 1.4Å" /> '''Kid toxin protein fro...
 
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[[Image:1m1f.jpg|left|200px]]<br /><applet load="1m1f" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1m1f, resolution 1.4&Aring;" />
'''Kid toxin protein from E.coli plasmid R1'''<br />


==Overview==
==Kid toxin protein from E.coli plasmid R1==
We have determined the structure of Kid toxin protein from E. coli plasmid, R1 involved in stable plasmid inheritance by postsegregational killing of, plasmid-less daughter cells. Kid forms a two-component system with its, antagonist, Kis antitoxin. Our 1.4 A crystal structure of Kid reveals a, 2-fold symmetric dimer that closely resembles the DNA gyrase-inhibitory, toxin protein CcdB from E. coli F plasmid despite the lack of any notable, sequence similarity. Analysis of nontoxic mutants of Kid suggests a target, interaction interface associated with toxicity that is in marked contrast, to that proposed for CcdB. A possible region for interaction of Kid with, the antitoxin is proposed that overlaps with the target binding site and, may explain the mode of antitoxin action.
<StructureSection load='1m1f' size='340' side='right'caption='[[1m1f]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1m1f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M1F FirstGlance]. <br>
1M1F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M1F OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m1f OCA], [https://pdbe.org/1m1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m1f RCSB], [https://www.ebi.ac.uk/pdbsum/1m1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m1f ProSAT]</span></td></tr>
Structural and functional analysis of the kid toxin protein from E. coli plasmid R1., Hargreaves D, Santos-Sierra S, Giraldo R, Sabariegos-Jareno R, de la Cueva-Mendez G, Boelens R, Diaz-Orejas R, Rafferty JB, Structure. 2002 Oct;10(10):1425-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12377128 12377128]
</table>
== Function ==
[https://www.uniprot.org/uniprot/PEMK_ECOLX PEMK_ECOLX] Toxic component of a type II toxin-antitoxin (TA) module. Probably functions as an endoribonuclease. Responsible for the stable maintenance of the plasmid during cell division by postsegregational killing of plasmid-less daughter cells. Neutralized by coexpression with cognate antitoxin PemI. Both PemI and PemK proteins bind to the promoter region of the pem operon to autoregulate their synthesis.<ref>PMID:2832364</ref> <ref>PMID:8455570</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m1/1m1f_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m1f ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Boelens, R.]]
[[Category: Boelens R]]
[[Category: Cueva-Mendez, G.de.la.]]
[[Category: Diaz-Orejas R]]
[[Category: Diaz-Orejas, R.]]
[[Category: Giraldo R]]
[[Category: Giraldo, R.]]
[[Category: Hargreaves D]]
[[Category: Hargreaves, D.]]
[[Category: Rafferty JB]]
[[Category: Rafferty, J.B.]]
[[Category: Sabariegos-Jareno R]]
[[Category: Sabariegos-Jareno, R.]]
[[Category: Santos-Sierra S]]
[[Category: Santos-Sierra, S.]]
[[Category: De la Cueva-Mendez G]]
[[Category: PO4]]
[[Category: ccdb]]
[[Category: dna replication]]
[[Category: mutational analysis]]
[[Category: plasmid maintenance]]
[[Category: post segregational killing]]
[[Category: toxin-antitoxin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:03:37 2007''

Latest revision as of 07:38, 14 February 2024

Kid toxin protein from E.coli plasmid R1

1m1f, resolution 1.40Å

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