1p6u: Difference between revisions

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{{Seed}}
[[Image:1p6u.png|left|200px]]


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==NMR structure of the BeF3-activated structure of the response regulator Chey2-Mg2+ from Sinorhizobium meliloti==
The line below this paragraph, containing "STRUCTURE_1p6u", creates the "Structure Box" on the page.
<StructureSection load='1p6u' size='340' side='right'caption='[[1p6u]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1p6u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P6U FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p6u OCA], [https://pdbe.org/1p6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p6u RCSB], [https://www.ebi.ac.uk/pdbsum/1p6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p6u ProSAT]</span></td></tr>
{{STRUCTURE_1p6u|  PDB=1p6u  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q52884_RHIML Q52884_RHIML]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/1p6u_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p6u ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The chemotactic signalling chain to the flagellar motor of Sinorhizobium meliloti features a new type of response regulator, CheY2. CheY2 activated by phosphorylation (CheY2-P) controls the rotary speed of the flagellar motor (instead of reversing the sense of rotation), and it is efficiently dephosphorylated by phospho-retrotransfer to the cognate kinase, CheA. Here, we report the NMR solution structures of the Mg(2+)-complex of inactive CheY2, and of activated CheY2-BeF(3), a stable analogue of CheY2-P, to an overall root mean square deviation of 0.042 nm and 0.027 nm, respectively. The 14 kDa CheY2 protein exhibits a characteristic open (alpha/beta)(5) conformation. Modification of CheY2 by BeF(3)(-) leads to large conformational changes of the protein, which are in the limits of error identical with those observed by phosphorylation of the active-centre residue Asp58. In BeF(3)-activated CheY2, the position of Thr88-OH favours the formation of a hydrogen bond with the active site, Asp58-BeF(3), similar to BeF(3)-activated CheY from Escherichia coli. In contrast to E.coli, this reorientation is not involved in a Tyr-Thr-coupling mechanism, that propagates the signal from the incoming phosphoryl group to the C-terminally located FliM-binding surface. Rather, a rearrangement of the Phe59 side-chain to interact with Ile86-Leu95-Val96 along with a displacement of alpha4 towards beta5 is stabilised in S.meliloti. The resulting, activation-induced, compact alpha4-beta5-alpha5 surface forms a unique binding domain suited for specific interaction with and signalling to a rotary motor that requires a gradual speed control. We propose that these new features of response regulator activation, compared to other two-component systems, are the key for the observed unique phosphorylation, dephosphorylation and motor control mechanisms in S.meliloti.


===NMR structure of the BeF3-activated structure of the response regulator Chey2-Mg2+ from Sinorhizobium meliloti===
Solution structures of the inactive and BeF3-activated response regulator CheY2.,Riepl H, Scharf B, Schmitt R, Kalbitzer HR, Maurer T J Mol Biol. 2004 Apr 23;338(2):287-97. PMID:15066432<ref>PMID:15066432</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_15066432}}, adds the Publication Abstract to the page
<div class="pdbe-citations 1p6u" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 15066432 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_15066432}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
1P6U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6U OCA].
 
==Reference==
Solution structures of the inactive and BeF3-activated response regulator CheY2., Riepl H, Scharf B, Schmitt R, Kalbitzer HR, Maurer T, J Mol Biol. 2004 Apr 23;338(2):287-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15066432 15066432]
[[Category: Single protein]]
[[Category: Sinorhizobium meliloti]]
[[Category: Sinorhizobium meliloti]]
[[Category: Kalbitzer, H R.]]
[[Category: Kalbitzer HR]]
[[Category: Maurer, T.]]
[[Category: Maurer T]]
[[Category: Riepl, H.]]
[[Category: Riepl H]]
[[Category: SPINE, Structural Proteomics in Europe.]]
[[Category: Scharf B]]
[[Category: Scharf, B.]]
[[Category: Schmitt R]]
[[Category: Schmitt, R.]]
[[Category: Activation]]
[[Category: Chemotaxis]]
[[Category: Chey2 beryllium fluoride]]
[[Category: Response regulator]]
[[Category: Signal transduction]]
[[Category: Spine]]
[[Category: Structural genomic]]
[[Category: Structural proteomics in europe]]
 
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Retrieved from "https://proteopedia.org/1p6u"