1mjz: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
New page: left|200px<br /><applet load="1mjz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mjz, resolution 2.2Å" /> '''STRUCTURE OF INORGANI...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(16 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1mjz.gif|left|200px]]<br /><applet load="1mjz" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1mjz, resolution 2.2&Aring;" />
'''STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D97N'''<br />


==Overview==
==STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D97N==
The three-dimensional structures of four mutant E. coli inorganic, pyrophosphatases (PPases) with single Asp--&gt;Asn substitutions at positions, 42, 65, 70, and 97 were solved at 1.95, 2.15, 2.10, and 2.20 A resolution, respectively. Asp-42--&gt;Asn and Asp-65--&gt;Asn mutant PPases were prepared as, complexes with sulfate--a structural analog of phosphate, the product of, enzymatic reaction. A comparison of mutant enzymes with native PPases, revealed that a single amino acid substitution changes the position of the, mutated residue as well as the positions of several functional groups and, some parts of a polypeptide chain. These changes are responsible for the, fact that mutant PPases differ from the native ones in their catalytic, properties. The sulfate binding to the mutant PPase active site causes, molecular asymmetry, as shown for the native PPase earlier. The subunit, asymmetry is manifested in different positions of sulfate and several, functional groups, as well as changes in packing of hexamers in crystals, and in cell parameters.
<StructureSection load='1mjz' size='340' side='right'caption='[[1mjz]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mjz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjz OCA], [https://pdbe.org/1mjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mjz RCSB], [https://www.ebi.ac.uk/pdbsum/1mjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mjz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IPYR_ECOLI IPYR_ECOLI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mj/1mjz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mjz ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MJZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MJZ OCA].
*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Three-dimensional structures of mutant forms of E. coli inorganic pyrophosphatase with Asp--&gt;Asn single substitution in positions 42, 65, 70, and 97., Avaeva SM, Rodina EV, Vorobyeva NN, Kurilova SA, Nazarova TI, Sklyankina VA, Oganessyan VY, Samygina VR, Harutyunyan EH, Biochemistry (Mosc). 1998 Jun;63(6):671-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9668207 9668207]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Inorganic diphosphatase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Avaeva SM]]
[[Category: Avaeva, S.M.]]
[[Category: Harutyunyan EH]]
[[Category: Harutyunyan, E.H.]]
[[Category: Huber R]]
[[Category: Huber, R.]]
[[Category: Oganesyan V]]
[[Category: Oganesyan, V.]]
[[Category: acid anhydride hydrolase]]
[[Category: hydrolase]]
[[Category: mutation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:28:10 2007''

Latest revision as of 07:44, 14 February 2024

STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D97N

1mjz, resolution 2.20Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1mjz&oldid=4051600"