1mpd: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="1mpd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mpd, resolution 2.3Å" /> '''MALTODEXTRIN-BINDING ...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1mpd.jpg|left|200px]]<br /><applet load="1mpd" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1mpd, resolution 2.3&Aring;" />
'''MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 (TRP-230-ARG), COMPLEXED WITH MALTOSE'''<br />


==Overview==
==MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 (TRP-230-ARG), COMPLEXED WITH MALTOSE==
A mutant of the periplasmic maltose-binding protein (MBP) with altered, transport properties was studied. A change of residue 230 from tryptophan, to arginine results in dominant-negative MBP: expression of this protein, against a wild-type background causes inhibition of maltose transport. As, part of an investigation of the mechanism of such inhibition, we have, solved crystal structures of both unliganded and liganded mutant protein., In the closed, liganded conformation, the side-chain of R230 projects into, a region of the surface of MBP that has been identified as important for, transport while in the open form, the same side-chain takes on a, different, and less ordered, conformation. The crystallographic work is, supplemented with a small-angle X-ray scattering study that provides, evidence that the solution conformation of unliganded mutant is similar to, that of wild-type MBP. It is concluded that dominant-negative inhibition, of maltose transport must result from the formation of a non-productive, complex between liganded-bound mutant MBP and wild-type MalFGK2. A general, kinetic framework for transport by either wild-type MalFGK2 or, MBP-independent MalFGK2 is used to understand the effects of, dominant-negative MBP molecules on both of these systems.
<StructureSection load='1mpd' size='340' side='right'caption='[[1mpd]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mpd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MPD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MPD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mpd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mpd OCA], [https://pdbe.org/1mpd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mpd RCSB], [https://www.ebi.ac.uk/pdbsum/1mpd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mpd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mpd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mpd ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MPD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MPD OCA].
*[[Maltose-binding protein 3D structures|Maltose-binding protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structures and solution conformations of a dominant-negative mutant of Escherichia coli maltose-binding protein., Shilton BH, Shuman HA, Mowbray SL, J Mol Biol. 1996 Nov 29;264(2):364-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8951382 8951382]
[[Category: Escherichia coli K-12]]
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Mowbray SL]]
[[Category: Mowbray, S.L.]]
[[Category: Shilton BH]]
[[Category: Shilton, B.H.]]
[[Category: MAL]]
[[Category: periplasmic binding protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:36:20 2007''

Latest revision as of 07:46, 14 February 2024

MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 (TRP-230-ARG), COMPLEXED WITH MALTOSE

1mpd, resolution 2.30Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1mpd&oldid=4051757"