1mpg: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="1mpg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mpg, resolution 1.80Å" /> '''3-METHYLADENINE DNA ...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1mpg.gif|left|200px]]<br /><applet load="1mpg" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1mpg, resolution 1.80&Aring;" />
'''3-METHYLADENINE DNA GLYCOSYLASE II FROM ESCHERICHIA COLI'''<br />


==Overview==
==3-METHYLADENINE DNA GLYCOSYLASE II FROM ESCHERICHIA COLI==
Base-excision DNA repair proteins that target alkylation damage act on a, variety of seemingly dissimilar adducts, yet fail to recognize other, closely related lesions. The 1.8 A crystal structure of the monofunctional, DNA glycosylase AlkA (E. coli 3-methyladenine-DNA glycosylase II) reveals, a large hydrophobic cleft unusually rich in aromatic residues. An Asp, residue projecting into this cleft is essential for catalysis, and it, governs binding specificity for mechanism-based inhibitors. We propose, that AlkA recognizes electron-deficient methylated bases through, pi-donor/acceptor interactions involving the electron-rich aromatic cleft., Remarkably, AlkA is similar in fold and active site location to the, bifunctional glycosylase/lyase endonuclease III, suggesting the two may, employ fundamentally related mechanisms for base excision.
<StructureSection load='1mpg' size='340' side='right'caption='[[1mpg]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mpg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MPG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mpg OCA], [https://pdbe.org/1mpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mpg RCSB], [https://www.ebi.ac.uk/pdbsum/1mpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mpg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/3MG2_ECOLI 3MG2_ECOLI] Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2-methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mpg_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mpg ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MPG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MPG OCA].
*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structural basis for the excision repair of alkylation-damaged DNA., Labahn J, Scharer OD, Long A, Ezaz-Nikpay K, Verdine GL, Ellenberger TE, Cell. 1996 Jul 26;86(2):321-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8706136 8706136]
[[Category: DNA-3-methyladenine glycosylase II]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Ellenberger, T.E.]]
[[Category: Ellenberger TE]]
[[Category: Ezaz-Nikpay, K.]]
[[Category: Ezaz-Nikpay K]]
[[Category: Labahn, J.]]
[[Category: Labahn J]]
[[Category: Long, A.]]
[[Category: Long A]]
[[Category: Schaerer, O.D.]]
[[Category: Schaerer OD]]
[[Category: Verdine, G.L.]]
[[Category: Verdine GL]]
[[Category: GOL]]
[[Category: alka]]
[[Category: base excision]]
[[Category: dna glycosylase]]
[[Category: dna repair]]
[[Category: hydrolase]]
[[Category: methylation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:36:32 2007''

Latest revision as of 07:47, 14 February 2024

3-METHYLADENINE DNA GLYCOSYLASE II FROM ESCHERICHIA COLI

1mpg, resolution 1.80Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1mpg&oldid=4051760"