2ote: Difference between revisions

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{{Seed}}
[[Image:2ote.png|left|200px]]


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==Crystal structure of a monomeric cyan fluorescent protein in the photobleached state==
The line below this paragraph, containing "STRUCTURE_2ote", creates the "Structure Box" on the page.
<StructureSection load='2ote' size='340' side='right'caption='[[2ote]], [[Resolution|resolution]] 1.47&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2ote]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clavularia_sp. Clavularia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OTE FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.47&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>
{{STRUCTURE_2ote|  PDB=2ote  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ote FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ote OCA], [https://pdbe.org/2ote PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ote RCSB], [https://www.ebi.ac.uk/pdbsum/2ote PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ote ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GFPL_CLASP GFPL_CLASP]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/2ote_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ote ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Fluorescent protein (FP) variants that can be reversibly converted between fluorescent and nonfluorescent states have proven to be a catalyst for innovation in the field of fluorescence microscopy. However, the structural basis of the process remains poorly understood. High-resolution structures of a FP derived from Clavularia in both the fluorescent and the light-induced nonfluorescent states reveal that the rapid and complete loss of fluorescence observed upon illumination with 450-nm light results from cis-trans isomerization of the chromophore. The photoinduced change in configuration from the well ordered cis isomer to the highly nonplanar and disordered trans isomer is accompanied by a dramatic rearrangement of internal side chains. Taken together, the structures provide an explanation for the loss of fluorescence upon illumination, the slow light-independent recovery, and the rapid light-induced recovery of fluorescence. The fundamental mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs reported to date.


===Crystal structure of a monomeric cyan fluorescent protein in the photobleached state===
Structural basis for reversible photobleaching of a green fluorescent protein homologue.,Henderson JN, Ai HW, Campbell RE, Remington SJ Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6672-7. Epub 2007 Apr 9. PMID:17420458<ref>PMID:17420458</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ote" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_17420458}}, adds the Publication Abstract to the page
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 17420458 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_17420458}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Clavularia sp]]
2OTE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clavularia_sp. Clavularia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTE OCA].
[[Category: Large Structures]]
 
[[Category: Ai H]]
==Reference==
[[Category: Campbell RE]]
Structural basis for reversible photobleaching of a green fluorescent protein homologue., Henderson JN, Ai HW, Campbell RE, Remington SJ, Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6672-7. Epub 2007 Apr 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17420458 17420458]
[[Category: Henderson JN]]
[[Category: Clavularia sp.]]
[[Category: Remington SJ]]
[[Category: Single protein]]
[[Category: Ai, H.]]
[[Category: Campbell, R E.]]
[[Category: Henderson, J N.]]
[[Category: Remington, S J.]]
[[Category: Beta can]]
[[Category: Fluorescent protein]]
 
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Retrieved from "https://proteopedia.org/2ote"