1yod: Difference between revisions

<StructureSection load='1yod' size='340' side='right'caption='[[1yod]], [[Resolution|resolution]] 1.80&Aring;' scene=''>

<table><tr><td colspan='2'>[[1yod]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YOD FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yod OCA], [https://pdbe.org/1yod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yod RCSB], [https://www.ebi.ac.uk/pdbsum/1yod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yod ProSAT]</span></td></tr>

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Phospholamban (PLB) is a pentameric transmembrane protein that regulates the Ca(2+)-dependent ATPase SERCA2a in sarcoplasmic reticulum membranes. We previously described the computational design of a water-soluble variant of phospholamban, WSPLB, which reproduced many of the structural and functional properties of the native membrane-soluble protein. While the full-length WSPLB forms a pentamer in solution, a truncated variant forms very stable tetramers. To obtain insight into the tetramer-pentamer cytoplasmic switch, we solved the crystal structure of the truncated construct, WSPLB 21-52. This peptide has a heptad sequence repeat with Leu residues at a- and Ile at d-positions from residues 31-52. The crystal structure revealed that WSPLB 21-52 adopted an antiparallel tetrameric coiled coil. This topology contrasts with the parallel topology of an analogue of the coiled-coil of GCN4 with the same Leu(a) Ile(d) repeat. Analysis of these structures revealed how the nature of the partially exposed residues at e- and g-positions influence the topology formed by the bundle. We also constructed a model for the pentameric form of PLB using the coiled-coil parameters derived from a single monomer in the tetrameric structure. This model suggests that both buried and interfacial hydrogen bonds are important for stabilizing the parallel pentamer.

X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils.,Slovic AM, Stayrook SE, North B, Degrado WF J Mol Biol. 2005 May 6;348(3):777-87. PMID:15826670<ref>PMID:15826670</ref>

==See Also==

*[[Phospholamban|Phospholamban]]

== References ==

<references/>

</StructureSection>

[[Category: Large Structures]]

[[Category: DeGrado WF]]

[[Category: North B]]

[[Category: Slovic AM]]

[[Category: Stayrook SE]]