2dgl: Difference between revisions

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{{Seed}}
[[Image:2dgl.png|left|200px]]


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==Crystal structure of Escherichia coli GadB in complex with bromide==
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<StructureSection load='2dgl' size='340' side='right'caption='[[2dgl]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2dgl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DGL FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
{{STRUCTURE_2dgl|  PDB=2dgl  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dgl OCA], [https://pdbe.org/2dgl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dgl RCSB], [https://www.ebi.ac.uk/pdbsum/2dgl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dgl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DCEB_ECOLI DCEB_ECOLI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dg/2dgl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dgl ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Escherichia coli and other enterobacteria exploit the H+ -consuming reaction catalysed by glutamate decarboxylase to survive the stomach acidity before reaching the intestine. Here we show that chloride, extremely abundant in gastric secretions, is an allosteric activator producing a 10-fold increase in the decarboxylase activity at pH 5.6. Cooperativity and sensitivity to chloride were lost when the N-terminal 14 residues, involved in the formation of two triple-helix bundles, were deleted by mutagenesis. X-ray structures, obtained in the presence of the substrate analogue acetate, identified halide-binding sites at the base of each N-terminal helix, showed how halide binding is responsible for bundle stability and demonstrated that the interconversion between active and inactive forms of the enzyme is a stepwise process. We also discovered an entirely novel structure of the cofactor pyridoxal 5'-phosphate (aldamine) to be responsible for the reversibly inactivated enzyme. Our results link the entry of chloride ions, via the H+/Cl- exchange activities of ClC-ec1, to the trigger of the acid stress response in the cell when the intracellular proton concentration has not yet reached fatal values.


===Crystal structure of Escherichia coli GadB in complex with bromide===
Escherichia coli acid resistance: pH-sensing, activation by chloride and autoinhibition in GadB.,Gut H, Pennacchietti E, John RA, Bossa F, Capitani G, De Biase D, Grutter MG EMBO J. 2006 Jun 7;25(11):2643-51. Epub 2006 May 4. PMID:16675957<ref>PMID:16675957</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_16675957}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2dgl" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 16675957 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_16675957}}
__TOC__
 
</StructureSection>
==About this Structure==
2DGL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DGL OCA].
 
==Reference==
Escherichia coli acid resistance: pH-sensing, activation by chloride and autoinhibition in GadB., Gut H, Pennacchietti E, John RA, Bossa F, Capitani G, De Biase D, Grutter MG, EMBO J. 2006 Jun 7;25(11):2643-51. Epub 2006 May 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16675957 16675957]
 
Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase., Capitani G, De Biase D, Aurizi C, Gut H, Bossa F, Grutter MG, EMBO J. 2003 Aug 15;22(16):4027-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12912902 12912902]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Glutamate decarboxylase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Capitani G]]
[[Category: Capitani, G.]]
[[Category: Gruetter MG]]
[[Category: Gruetter, M G.]]
[[Category: Gut H]]
[[Category: Gut, H.]]
[[Category: Gadb complexed with bromide]]
 
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Retrieved from "https://proteopedia.org/2dgl"