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New page: left|200px<br /><applet load="1mvx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mvx, resolution 3.0Å" /> '''structure of the SET ...
 
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[[Image:1mvx.jpg|left|200px]]<br /><applet load="1mvx" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1mvx, resolution 3.0&Aring;" />
'''structure of the SET domain histone lysine methyltransferase Clr4'''<br />


==Overview==
==structure of the SET domain histone lysine methyltransferase Clr4==
Methylation of histone H3 lysine 9 is an important component of the, 'histone code' for heterochromatic gene silencing. The SET, domain-containing Clr4 protein, a close relative of Su(var)3-9 proteins in, higher eukaryotes, specifically methylates lysine 9 of histone H3 and is, essential for silencing in Schizosaccharomyces pombe. Here we report the, 2.3 A resolution crystal structure of the catalytic domain of Clr4. The, structure reveals an overall fold rich in beta-strands, a potential active, site consisting of a SAM-binding pocket, and a connected groove that could, accommodate the binding of the N-terminal tail of histone H3. The pre-SET, motif contains a triangular zinc cluster coordinated by nine cysteines, distant from the active site, whereas the post-SET region is largely, flexible but proximal to the active site. The structure provides insights, into the architecture of SET domain histone methyltransferases and, establishes a paradigm for further characterization of the Clr4 family of, epigenetic regulators.
<StructureSection load='1mvx' size='340' side='right'caption='[[1mvx]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1mvx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVX FirstGlance]. <br>
1MVX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with SO4, ZN and NI as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MVX OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvx OCA], [https://pdbe.org/1mvx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvx RCSB], [https://www.ebi.ac.uk/pdbsum/1mvx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvx ProSAT]</span></td></tr>
Structure of the SET domain histone lysine methyltransferase Clr4., Min J, Zhang X, Cheng X, Grewal SI, Xu RM, Nat Struct Biol. 2002 Nov;9(11):828-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12389037 12389037]
</table>
== Function ==
[https://www.uniprot.org/uniprot/CLR4_SCHPO CLR4_SCHPO] Histone methyltransferase. Catalytic component of the rik1-associated E3 ubiquitin ligase complex that shows ubiquitin ligase activity and is required for histone H3K9 methylation. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting swi6/HP1 to methylated histones which leads to transcriptional silencing within centromeric heterochromatin, telomeric regions and at the silent mating-type loci.<ref>PMID:16024659</ref> <ref>PMID:8138176</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/1mvx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mvx ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Single protein]]
[[Category: Cheng XD]]
[[Category: Cheng, X.D.]]
[[Category: Grewal SIS]]
[[Category: Grewal, S.I.S.]]
[[Category: Min JR]]
[[Category: Min, J.R.]]
[[Category: Xu R-M]]
[[Category: Xu, R.M.]]
[[Category: Zhang X]]
[[Category: Zhang, X.]]
[[Category: NI]]
[[Category: SO4]]
[[Category: ZN]]
[[Category: clr4]]
[[Category: lysine methyltransferase]]
[[Category: set-domain]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:44:43 2007''

Latest revision as of 07:49, 14 February 2024

structure of the SET domain histone lysine methyltransferase Clr4

1mvx, resolution 3.00Å

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