1mwm: Difference between revisions

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New page: left|200px<br /><applet load="1mwm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mwm, resolution 2.0Å" /> '''ParM from plasmid R1 ...
 
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[[Image:1mwm.gif|left|200px]]<br /><applet load="1mwm" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1mwm, resolution 2.0&Aring;" />
'''ParM from plasmid R1 ADP form'''<br />


==Overview==
==ParM from plasmid R1 ADP form==
It was the general belief that DNA partitioning in prokaryotes is, independent of a cytoskeletal structure, which in eukaryotic cells is, indispensable for DNA segregation. Recently, however, immunofluorescence, microscopy revealed highly dynamic, filamentous structures along the, longitudinal axis of Escherichia coli formed by ParM, a plasmid-encoded, protein required for accurate segregation of low-copy-number plasmid R1., We show here that ParM polymerizes into double helical protofilaments with, a longitudinal repeat similar to filamentous actin (F-actin) and MreB, filaments that maintain the cell shape of non-spherical bacteria. The, crystal structure of ParM with and without ADP demonstrates that it is a, member of the actin family of proteins and shows a domain movement of 25, degrees upon nucleotide binding. Furthermore, the crystal structure of, ParM reveals major differences in the protofilament interface compared, with F-actin, despite the similar arrangement of the subunits within the, filaments. Thus, there is now evidence for cytoskeletal structures, formed, by actin-like filaments that are involved in plasmid partitioning in, E.coli.
<StructureSection load='1mwm' size='340' side='right'caption='[[1mwm]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mwm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwm OCA], [https://pdbe.org/1mwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwm RCSB], [https://www.ebi.ac.uk/pdbsum/1mwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PARM_ECOLX PARM_ECOLX] Involved in the control of plasmid partition. Required for the accurate segregation of the plasmid.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mwm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mwm ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MWM OCA].
*[[Plasmid segregation protein ParM|Plasmid segregation protein ParM]]
 
__TOC__
==Reference==
</StructureSection>
F-actin-like filaments formed by plasmid segregation protein ParM., van den Ent F, Moller-Jensen J, Amos LA, Gerdes K, Lowe J, EMBO J. 2002 Dec 16;21(24):6935-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12486014 12486014]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Amos, L.A.]]
[[Category: Amos LA]]
[[Category: Ent, F.Van.den.]]
[[Category: Gerdes K]]
[[Category: Gerdes, K.]]
[[Category: Lowe J]]
[[Category: Lowe, J.]]
[[Category: Moller-Jensen J]]
[[Category: Moller-Jensen, J.]]
[[Category: Van den Ent F]]
[[Category: ADP]]
[[Category: MG]]
[[Category: parm]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:46:03 2007''

Latest revision as of 13:27, 13 March 2024

ParM from plasmid R1 ADP form

1mwm, resolution 2.00Å

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