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New page: left|200px<br /><applet load="1n1h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n1h, resolution 2.80Å" /> '''Initiation complex o...
 
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[[Image:1n1h.gif|left|200px]]<br /><applet load="1n1h" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1n1h, resolution 2.80&Aring;" />
'''Initiation complex of polymerase lambda3 from reovirus'''<br />


==Overview==
==Initiation complex of polymerase lambda3 from reovirus==
The reovirus polymerase and those of other dsRNA viruses function within, the confines of a protein capsid to transcribe the tightly packed dsRNA, genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and, C-terminal elaborations, which create a cage-like structure, with four, channels leading to the catalytic site. This "caged" polymerase has, allowed us to visualize the results of several rounds of RNA, polymerization directly in the crystals. A 5' cap binding site on the, surface of lambda3 suggests a template retention mechanism by which, attachment of the 5' end of the plus-sense strand facilitates insertion of, the 3' end of the minus-sense strand into the template channel.
<StructureSection load='1n1h' size='340' side='right'caption='[[1n1h]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1n1h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_Dearing Mammalian orthoreovirus 3 Dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N1H FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CH1:3-DEOXY-CYTIDINE-5-TRIPHOSPHATE'>CH1</scene>, <scene name='pdbligand=G7M:N7-METHYL-GUANOSINE-5-MONOPHOSPHATE'>G7M</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GH3:3-DEOXY-GUANOSINE-5-TRIPHOSPHATE'>GH3</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n1h OCA], [https://pdbe.org/1n1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n1h RCSB], [https://www.ebi.ac.uk/pdbsum/1n1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n1h ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RDRP_REOVD RDRP_REOVD] RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.


==About this Structure==
==See Also==
1N1H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3 Mammalian orthoreovirus 3] with MN, G7M, GDP, CH1 and GH3 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N1H OCA].
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
RNA synthesis in a cage--structural studies of reovirus polymerase lambda3., Tao Y, Farsetta DL, Nibert ML, Harrison SC, Cell. 2002 Nov 27;111(5):733-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12464184 12464184]
[[Category: Large Structures]]
[[Category: Mammalian orthoreovirus 3]]
[[Category: Mammalian orthoreovirus 3 Dearing]]
[[Category: Single protein]]
[[Category: Farsetta DL]]
[[Category: Farsetta, D.L.]]
[[Category: Harrison SC]]
[[Category: Harrison, S.C.]]
[[Category: Nibert ML]]
[[Category: Nibert, M.L.]]
[[Category: Tao Y]]
[[Category: Tao, Y.]]
[[Category: CH1]]
[[Category: G7M]]
[[Category: GDP]]
[[Category: GH3]]
[[Category: MN]]
[[Category: initiation complex]]
[[Category: polymerase]]
[[Category: right hand configuration]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:52:22 2007''

Latest revision as of 07:51, 14 February 2024

Initiation complex of polymerase lambda3 from reovirus

1n1h, resolution 2.80Å

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