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New page: left|200px<br /><applet load="1n22" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n22, resolution 2.40Å" /> '''(+)-Bornyl Diphospha...
 
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[[Image:1n22.gif|left|200px]]<br /><applet load="1n22" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1n22, resolution 2.40&Aring;" />
'''(+)-Bornyl Diphosphate Synthase: Complex with Mg, pyrophosphate, and (4R)-7-aza-7,8-dihydrolimonene'''<br />


==Overview==
==(+)-Bornyl Diphosphate Synthase: Complex with Mg, pyrophosphate, and (4R)-7-aza-7,8-dihydrolimonene==
The x-ray crystal structure of dimeric (+)-bornyl diphosphate synthase, a, metal-requiring monoterpene cyclase from Salvia officinalis, is reported, at 2.0-A resolution. Each monomer contains two alpha-helical domains: the, C-terminal domain catalyzes the cyclization of geranyl diphosphate, orienting and stabilizing multiple reactive carbocation intermediates; the, N-terminal domain has no clearly defined function, although its N terminus, caps the active site in the C-terminal domain during catalysis. Structures, of complexes with aza analogues of substrate and carbocation, intermediates, as well as complexes with pyrophosphate and bornyl, diphosphate, provide "snapshots" of the terpene cyclization cascade.
<StructureSection load='1n22' size='340' side='right'caption='[[1n22]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1n22]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salvia_officinalis Salvia officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N22 FirstGlance]. <br>
1N22 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salvia_officinalis Salvia officinalis] with MG, 7A8 and POP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Geranyl-diphosphate_cyclase Geranyl-diphosphate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.8 5.5.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N22 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7A8:(1R)-DIMETHYL-(4-METHYL-CYCLOHEX-3-ENYL)-AMINE'>7A8</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n22 OCA], [https://pdbe.org/1n22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n22 RCSB], [https://www.ebi.ac.uk/pdbsum/1n22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n22 ProSAT]</span></td></tr>
Bornyl diphosphate synthase: structure and strategy for carbocation manipulation by a terpenoid cyclase., Whittington DA, Wise ML, Urbansky M, Coates RM, Croteau RB, Christianson DW, Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15375-80. Epub 2002 Nov 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12432096 12432096]
</table>
[[Category: Geranyl-diphosphate cyclase]]
== Function ==
[https://www.uniprot.org/uniprot/BPPS_SALOF BPPS_SALOF] Catalyzes the formation of the (+)-camphor precursor (+)-bornyl diphosphate from geranyl diphosphate. The enzyme also produces significant amounts of (+)-alpha-pinene, (+)-camphene, and (+-)-limonene.<ref>PMID:9614092</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/1n22_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n22 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Salvia officinalis]]
[[Category: Salvia officinalis]]
[[Category: Single protein]]
[[Category: Christianson DW]]
[[Category: Christianson, D.W.]]
[[Category: Coates RM]]
[[Category: Coates, R.M.]]
[[Category: Croteau RB]]
[[Category: Croteau, R.B.]]
[[Category: Urbansky M]]
[[Category: Urbansky, M.]]
[[Category: Whittington DA]]
[[Category: Whittington, D.A.]]
[[Category: Wise ML]]
[[Category: Wise, M.L.]]
[[Category: 7A8]]
[[Category: MG]]
[[Category: POP]]
[[Category: terpene synthase fold]]
 
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Retrieved from "https://proteopedia.org/1n22"