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New page: left|200px<br /><applet load="1n7e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n7e, resolution 1.50Å" /> '''Crystal structure of...
 
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[[Image:1n7e.jpg|left|200px]]<br /><applet load="1n7e" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1n7e, resolution 1.50&Aring;" />
'''Crystal structure of the sixth PDZ domain of GRIP1'''<br />


==Overview==
==Crystal structure of the sixth PDZ domain of GRIP1==
PDZ domains bind to short segments within target proteins in a, sequence-specific fashion. Glutamate receptor-interacting protein, (GRIP)/ABP family proteins contain six to seven PDZ domains and interact, via the sixth PDZ domain (class II) with the C termini of various proteins, including liprin-alpha. In addition the PDZ456 domain mediates the, formation of homo- and heteromultimers of GRIP proteins. To better, understand the structural basis of peptide recognition by a class II PDZ, domain and PDZ-mediated multimerization, we determined the crystal, structures of the GRIP1 PDZ6 domain alone and in complex with a synthetic, C-terminal octapeptide of human liprin-alpha at resolutions of 1.5 and 1.8, A, respectively. Remarkably, unlike other class II PDZ domains, Ile-736 at, alphaB5 rather than conserved Leu-732 at alphaB1 makes a direct, hydrophobic contact with the side chain of the Tyr at the -2 position of, the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight, reorientation of helix alphaB, indicating that the second hydrophobic, pocket undergoes a conformational adaptation to accommodate the bulkiness, of the Tyr side chain, and forms an antiparallel dimer through an, interface located at a site distal to the peptide-binding groove. This, configuration may enable formation of GRIP multimers and efficient, clustering of GRIP-binding proteins.
<StructureSection load='1n7e' size='340' side='right'caption='[[1n7e]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1n7e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N7E FirstGlance]. <br>
1N7E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N7E OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7e OCA], [https://pdbe.org/1n7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n7e RCSB], [https://www.ebi.ac.uk/pdbsum/1n7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7e ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of GRIP1 PDZ6-peptide complex reveals the structural basis for class II PDZ target recognition and PDZ domain-mediated multimerization., Im YJ, Park SH, Rho SH, Lee JH, Kang GB, Sheng M, Kim E, Eom SH, J Biol Chem. 2003 Mar 7;278(10):8501-7. Epub 2002 Dec 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12493751 12493751]
== Function ==
[https://www.uniprot.org/uniprot/GRIP1_RAT GRIP1_RAT] May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.<ref>PMID:9069286</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/1n7e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n7e ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Eom SH]]
[[Category: Eom, S.H.]]
[[Category: Im YJ]]
[[Category: Im, Y.J.]]
[[Category: Kang GB]]
[[Category: Kang, G.B.]]
[[Category: Kim E]]
[[Category: Kim, E.]]
[[Category: Lee JH]]
[[Category: Lee, J.H.]]
[[Category: Park SH]]
[[Category: Park, S.H.]]
[[Category: Rho SH]]
[[Category: Rho, S.H.]]
[[Category: Sheng M]]
[[Category: Sheng, M.]]
[[Category: grip]]
[[Category: pdz]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:01:06 2007''

Latest revision as of 13:27, 13 March 2024

Crystal structure of the sixth PDZ domain of GRIP1

1n7e, resolution 1.50Å

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