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New page: left|200px<br /><applet load="1nfj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nfj, resolution 2.0Å" /> '''Structure of a Sir2 s...
 
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[[Image:1nfj.gif|left|200px]]<br /><applet load="1nfj" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1nfj, resolution 2.0&Aring;" />
'''Structure of a Sir2 substrate, alba, reveals a mechanism for deactylation-induced enhancement of DNA-binding'''<br />


==Overview==
==Structure of a Sir2 substrate, alba, reveals a mechanism for deactylation-induced enhancement of DNA-binding==
The targeted acetylation status of histones and several other, transcriptional regulatory proteins plays an important role in gene, expression, although the mechanism for this is not well understood. As a, model to understand how targeted acetylation may effect transcription, we, determined the x-ray crystal structure of the chromatin protein Alba from, Archaeoglobus fulgidus, a substrate for the Sir2 protein that deacetylates, it at lysine 11 to promote DNA binding by Alba. The structure reveals a, dimer of dimers in which the dimer-dimer interface is stabilized by, several conserved hydrophobic residues as well as the lysine 11 target of, Sir2. We show that, in solution, the mutation of these hydrophobic, residues or lysine 11 disrupts dimer-dimer formation and decreases, DNA-binding affinity. We propose that the in vivo deacetylation of lysine, 11 of archaeal Alba by Sir2 promotes protein oligomerization for optimal, DNA binding. Implications for the mechanism by which histone acetylation, modulates gene expression are discussed.
<StructureSection load='1nfj' size='340' side='right'caption='[[1nfj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1nfj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NFJ FirstGlance]. <br>
1NFJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NFJ OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nfj OCA], [https://pdbe.org/1nfj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nfj RCSB], [https://www.ebi.ac.uk/pdbsum/1nfj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nfj ProSAT]</span></td></tr>
==Reference==
</table>
Structure of a Sir2 substrate, Alba, reveals a mechanism for deacetylation-induced enhancement of DNA binding., Zhao K, Chai X, Marmorstein R, J Biol Chem. 2003 Jul 11;278(28):26071-7. Epub 2003 May 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12730210 12730210]
== Function ==
[https://www.uniprot.org/uniprot/ALBA2_ARCFU ALBA2_ARCFU] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes (By similarity).[HAMAP-Rule:MF_01122]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nf/1nfj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nfj ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Chai, X.]]
[[Category: Chai X]]
[[Category: Marmorstein, R.]]
[[Category: Marmorstein R]]
[[Category: Zhao, K.]]
[[Category: Zhao K]]
[[Category: alba]]
[[Category: gene regulation]]
[[Category: hdac]]
[[Category: sir2]]
[[Category: transcription]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:12:53 2007''

Latest revision as of 07:56, 14 February 2024

Structure of a Sir2 substrate, alba, reveals a mechanism for deactylation-induced enhancement of DNA-binding

1nfj, resolution 2.00Å

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