1ni8: Difference between revisions

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{{Seed}}
[[Image:1ni8.png|left|200px]]


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==H-NS dimerization motif==
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<StructureSection load='1ni8' size='340' side='right'caption='[[1ni8]]' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1ni8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NI8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NI8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ni8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ni8 OCA], [https://pdbe.org/1ni8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ni8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ni8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ni8 ProSAT]</span></td></tr>
{{STRUCTURE_1ni8|  PDB=1ni8  |  SCENE=  }}
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== Function ==
[https://www.uniprot.org/uniprot/HNS_ECOLI HNS_ECOLI] A DNA-binding protein implicated in transcriptional repression (silencing) as well as in bacterial chromosome organization. H-NS binds tightly to AT-rich dsDNA, increases its thermal stability and inhibits transcription. Also binds to ssDNA and RNA but with a much lower affinity. H-NS has possible histone-like function. May be a global transcriptional regulator through its ability to bind to curved DNA sequences, which are found in regions upstream of a certain subset of promoters. Plays a role in the thermal control of pili and adhesive curli fimbriae production, by inducing transcription of csgD. Represses the CRISPR-cas promoters, permits only weak transcription of the crRNA precursor; its role is antagonized by LeuO. Subject to transcriptional auto-repression. Binds preferentially to the upstream region of its own gene recognizing two segments of DNA on both sides of a bend centered around -150.<ref>PMID:7934818</ref> <ref>PMID:11031114</ref> <ref>PMID:17010156</ref> <ref>PMID:20659289</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
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    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ni/1ni8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ni8 ConSurf].
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== Publication Abstract from PubMed ==
H-NS, a protein found in Gram-negative bacteria, is involved in structuring the bacterial chromosome and acts as a global regulator for the expression of a wide variety of genes. These functions are correlated with both its DNA-binding and oligomerization properties. We have identified the minimal dimerization domain of H-NS, a 46 amino acid-long N-terminal fragment, and determined its structure using heteronuclear NMR spectroscopy. The highly intertwined structure of the dimer, reminiscent of a handshake, defines a new structural fold, which may offer a possibility for discriminating prokaryotic from eukaryotic proteins in drug design. Using mutational analysis, we also show that this N-terminal domain actively contributes to DNA binding, conversely to the current paradigm. Together, our data allows us to propose a model for the action of full length H-NS.


===H-NS dimerization motif===
The H-NS dimerization domain defines a new fold contributing to DNA recognition.,Bloch V, Yang Y, Margeat E, Chavanieu A, Auge MT, Robert B, Arold S, Rimsky S, Kochoyan M Nat Struct Biol. 2003 Mar;10(3):212-8. PMID:12592399<ref>PMID:12592399</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 12592399 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_12592399}}
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</StructureSection>
==About this Structure==
1NI8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NI8 OCA].
 
==Reference==
The H-NS dimerization domain defines a new fold contributing to DNA recognition., Bloch V, Yang Y, Margeat E, Chavanieu A, Auge MT, Robert B, Arold S, Rimsky S, Kochoyan M, Nat Struct Biol. 2003 Mar;10(3):212-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12592399 12592399]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Arold, S.]]
[[Category: Arold S]]
[[Category: Aug, M T.]]
[[Category: Aug MT]]
[[Category: Bloch, V.]]
[[Category: Bloch V]]
[[Category: Chavanieu, A.]]
[[Category: Chavanieu A]]
[[Category: Kochoyan, M.]]
[[Category: Kochoyan M]]
[[Category: Margeat, E.]]
[[Category: Margeat E]]
[[Category: Rimsky, S.]]
[[Category: Rimsky S]]
[[Category: Robert, B.]]
[[Category: Robert B]]
[[Category: Yang, Y.]]
[[Category: Yang Y]]
[[Category: Dimerization]]
[[Category: Protein-dna interaction]]
 
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Retrieved from "https://proteopedia.org/1ni8"