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New page: left|200px<br /><applet load="1nlq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nlq, resolution 1.50Å" /> '''The crystal structur...
 
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[[Image:1nlq.jpg|left|200px]]<br /><applet load="1nlq" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1nlq, resolution 1.50&Aring;" />
'''The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding'''<br />


==Overview==
==The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding==
The nucleoplasmin-like protein from Drosophila (dNLP) functions as a, chaperone for core histones and may remodel chromatin in embryos. We now, report the crystal structure of a dNLP-core pentamer at 1.5 A resolution., The monomer has an eight-stranded, beta barrel topology that is similar to, nucleoplasmin (Np). However, a signature beta hairpin is tucked in along, the lateral surface of the dNLP-core pentamer, while it extends outward in, the Np-core decamer. Drosophila NLP and Np both assemble histone octamers., This process may require each chaperone to form a decamer, which would, create symmetric binding sites for the histones. Conformational, differences between dNLP and Np may reflect their different oligomeric, states, while a conserved, nonpolar subunit interface may allow, conformational plasticity during histone binding.
<StructureSection load='1nlq' size='340' side='right'caption='[[1nlq]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1nlq]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NLQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NLQ FirstGlance]. <br>
1NLQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NLQ OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nlq OCA], [https://pdbe.org/1nlq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nlq RCSB], [https://www.ebi.ac.uk/pdbsum/1nlq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nlq ProSAT]</span></td></tr>
The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding., Namboodiri VM, Dutta S, Akey IV, Head JF, Akey CW, Structure. 2003 Feb;11(2):175-86. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12575937 12575937]
</table>
== Function ==
[https://www.uniprot.org/uniprot/NLP_DROME NLP_DROME] Binds to core histones and functions in the ATP-facilitated assembly of approximately regularly spaced nucleosomal arrays. May participate in parallel with other histone-binding proteins such as NAP-1.<ref>PMID:8798787</ref> <ref>PMID:9087911</ref>  Isoform 2 is inactive for chromatin assembly. In vitro it appears to form a high molecular mass aggregate with the core histones.<ref>PMID:8798787</ref> <ref>PMID:9087911</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nl/1nlq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nlq ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Akey, C.W.]]
[[Category: Akey CW]]
[[Category: Akey, I.V.]]
[[Category: Akey IV]]
[[Category: Dutta, S.]]
[[Category: Dutta S]]
[[Category: Head, J.F.]]
[[Category: Head JF]]
[[Category: Namboodiri, V.M.H.]]
[[Category: Namboodiri VMH]]
[[Category: MG]]
[[Category: chaperone]]
[[Category: dnlp]]
[[Category: histone binding]]
[[Category: nucleoplasmin]]
[[Category: x-ray crystallography]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:22:23 2007''

Latest revision as of 07:57, 14 February 2024

The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding

1nlq, resolution 1.50Å

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