2o1i: Difference between revisions

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{{Seed}}
[[Image:2o1i.png|left|200px]]


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==RH(BPY)2CHRYSI complexed to mismatched DNA==
The line below this paragraph, containing "STRUCTURE_2o1i", creates the "Structure Box" on the page.
<StructureSection load='2o1i' size='340' side='right'caption='[[2o1i]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2o1i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O1I FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=R1C:BIS(2,2-BIPYRIDINE-KAPPA~2~N~1~,N~1~)[CHRYSENE-5,6-DIIMINATO(2-)-KAPPA~2~N,N]RHODIUM(4+)'>R1C</scene></td></tr>
{{STRUCTURE_2o1i|  PDB=2o1i  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o1i OCA], [https://pdbe.org/2o1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o1i RCSB], [https://www.ebi.ac.uk/pdbsum/2o1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o1i ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report the 1.1-A resolution crystal structure of a bulky rhodium complex bound to two different DNA sites, mismatched and matched in the oligonucleotide 5'-(dCGGAAATTCCCG)2-3'. At the AC mismatch site, the structure reveals ligand insertion from the minor groove with ejection of both mismatched bases and elucidates how destabilized mispairs in DNA may be recognized. This unique binding mode contrasts with major groove intercalation, observed at a matched site, where doubling of the base pair rise accommodates stacking of the intercalator. Mass spectral analysis reveals different photocleavage products associated with the two binding modes in the crystal, with only products characteristic of mismatch binding in solution. This structure, illustrating two clearly distinct binding modes for a molecule with DNA, provides a rationale for the interrogation and detection of mismatches.


===RH(BPY)2CHRYSI complexed to mismatched DNA===
Insights into finding a mismatch through the structure of a mispaired DNA bound by a rhodium intercalator.,Pierre VC, Kaiser JT, Barton JK Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):429-34. Epub 2006 Dec 28. PMID:17194756<ref>PMID:17194756</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_17194756}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17194756 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_17194756}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O1I OCA].
[[Category: Barton JK]]
 
[[Category: Kaiser JT]]
==Reference==
[[Category: Pierre VC]]
Insights into finding a mismatch through the structure of a mispaired DNA bound by a rhodium intercalator., Pierre VC, Kaiser JT, Barton JK, Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):429-34. Epub 2006 Dec 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17194756 17194756]
[[Category: Barton, J K.]]
[[Category: Kaiser, J T.]]
[[Category: Pierre, V C.]]
[[Category: Dna mismatch]]
[[Category: Dna recognition]]
[[Category: Metallointercalator]]
 
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