1npu: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
New page: left|200px<br /><applet load="1npu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1npu, resolution 2.00Å" /> '''CRYSTAL STRUCTURE OF...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(20 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1npu.gif|left|200px]]<br /><applet load="1npu" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1npu, resolution 2.00&Aring;" />
'''CRYSTAL STRUCTURE OF THE EXTRACELLULAR DOMAIN OF MURINE PD-1'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE EXTRACELLULAR DOMAIN OF MURINE PD-1==
PD-1, a member of the CD28/CTLA-4/ICOS costimulatory receptor family, delivers negative signals that have profound effects on T and B cell, immunity. The 2.0 A crystal structure of the extracellular domain of, murine PD-1 reveals an Ig V-type topology with overall similarity to the, CTLA-4 monomer; however, there are notable differences in regions relevant, to function. Our structural and biophysical data show that PD-1 is, monomeric both in solution as well as on cell surface, in contrast to, CTLA-4 and other family members that are all disulfide-linked homodimers., Furthermore, our structure-based mutagenesis studies identify the ligand, binding surface of PD-1, which displays significant differences compared, to those present in the other members of the family.
<StructureSection load='1npu' size='340' side='right'caption='[[1npu]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NPU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1npu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1npu OCA], [https://pdbe.org/1npu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1npu RCSB], [https://www.ebi.ac.uk/pdbsum/1npu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1npu ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1npu TOPSAN]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/1npu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1npu ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
PD-1, a member of the CD28/CTLA-4/ICOS costimulatory receptor family, delivers negative signals that have profound effects on T and B cell immunity. The 2.0 A crystal structure of the extracellular domain of murine PD-1 reveals an Ig V-type topology with overall similarity to the CTLA-4 monomer; however, there are notable differences in regions relevant to function. Our structural and biophysical data show that PD-1 is monomeric both in solution as well as on cell surface, in contrast to CTLA-4 and other family members that are all disulfide-linked homodimers. Furthermore, our structure-based mutagenesis studies identify the ligand binding surface of PD-1, which displays significant differences compared to those present in the other members of the family.


==About this Structure==
Structural and functional analysis of the costimulatory receptor programmed death-1.,Zhang X, Schwartz JC, Guo X, Bhatia S, Cao E, Lorenz M, Cammer M, Chen L, Zhang ZY, Edidin MA, Nathenson SG, Almo SC Immunity. 2004 Mar;20(3):337-47. PMID:15030777<ref>PMID:15030777</ref>
1NPU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NPU OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structural and functional analysis of the costimulatory receptor programmed death-1., Zhang X, Schwartz JC, Guo X, Bhatia S, Cao E, Lorenz M, Cammer M, Chen L, Zhang ZY, Edidin MA, Nathenson SG, Almo SC, Immunity. 2004 Mar;20(3):337-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15030777 15030777]
</div>
[[Category: Mus musculus]]
<div class="pdbe-citations 1npu" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
[[Category: Almo, S.C.]]
[[Category: Cao, E.]]
[[Category: Chen, L.]]
[[Category: Guo, X.]]
[[Category: NYSGXRC, New.York.Structural.GenomiX.Research.Consortium.]]
[[Category: Nathenson, S.G.]]
[[Category: Schwartz, J.C.D.]]
[[Category: Zhang, X.]]
[[Category: Zhang, Z.Y.]]
[[Category: ig v-type domain]]
[[Category: new york structural genomix research consortium]]
[[Category: nysgxrc]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: structural genomics]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:28:28 2007''
==See Also==
*[[Cell death protein 3D structures|Cell death protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Almo SC]]
[[Category: Burley SK]]
[[Category: Cao E]]
[[Category: Chen L]]
[[Category: Guo X]]
[[Category: Nathenson SG]]
[[Category: Schwartz J-CD]]
[[Category: Zhang X]]
[[Category: Zhang Z-Y]]
Retrieved from "https://proteopedia.org/1npu"