2gcb: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(10 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{Seed}}
[[Image:2gcb.png|left|200px]]


<!--
==G51S/S52T double mutant of L. casei FPGS==
The line below this paragraph, containing "STRUCTURE_2gcb", creates the "Structure Box" on the page.
<StructureSection load='2gcb' size='340' side='right'caption='[[2gcb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2gcb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lacticaseibacillus_casei Lacticaseibacillus casei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GCB FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gcb OCA], [https://pdbe.org/2gcb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gcb RCSB], [https://www.ebi.ac.uk/pdbsum/2gcb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gcb ProSAT]</span></td></tr>
{{STRUCTURE_2gcb| PDB=2gcb |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/FPGS_LACCA FPGS_LACCA] Involved in the conversion of folates to polyglutamate derivatives, and likely functions in the retention of cellular folate pools. Catalyzes successive MgATP-dependent additions of glutamate to a pteroylmonoglutamate substrate, with a high preference for 5,10-methylenetetrahydrofolate (mTHF). Thus, metabolizes mTHF to the tetraglutamate derivative, but longer glutamate chain length products are not observed. Tetrahydrofolate (H4PteGlu) and 10-formyl-H4PteGlu are poorer folate substrates. In contrast to E.coli FolC, this enzyme does not display dihydrofolate synthase activity.<ref>PMID:18232714</ref> <ref>PMID:6138353</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gc/2gcb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gcb ConSurf].
<div style="clear:both"></div>


===G51S/S52T double mutant of L. casei FPGS===
==See Also==
 
*[[Folylpolyglutamate synthase|Folylpolyglutamate synthase]]
 
== References ==
<!--
<references/>
The line below this paragraph, {{ABSTRACT_PUBMED_16627949}}, adds the Publication Abstract to the page
__TOC__
(as it appears on PubMed at http://www.pubmed.gov), where 16627949 is the PubMed ID number.
</StructureSection>
-->
[[Category: Lacticaseibacillus casei]]
{{ABSTRACT_PUBMED_16627949}}
[[Category: Large Structures]]
 
[[Category: Bognar AL]]
==About this Structure==
[[Category: Cross JA]]
2GCB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_casei Lactobacillus casei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCB OCA].
[[Category: Smith CA]]
 
[[Category: Sun X]]
==Reference==
Mutation of Gly51 to serine in the P-loop of Lactobacillus casei folylpolyglutamate synthetase abolishes activity by altering the conformation of two adjacent loops., Smith CA, Cross JA, Bognar AL, Sun X, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):548-58. Epub 2006, Apr 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16627949 16627949]
[[Category: Lactobacillus casei]]
[[Category: Single protein]]
[[Category: Tetrahydrofolate synthase]]
[[Category: Bognar, A L.]]
[[Category: Cross, J A.]]
[[Category: Smith, C A.]]
[[Category: Sun, X.]]
[[Category: Atpase]]
[[Category: P-loop enzyme]]
[[Category: Site-directed mutant]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:46:56 2008''

Latest revision as of 09:27, 14 February 2024

G51S/S52T double mutant of L. casei FPGS

2gcb, resolution 2.30Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2gcb&oldid=4058656"