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New page: left|200px<br /><applet load="1nze" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nze, resolution 1.95Å" /> '''Crystal structure of...
 
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[[Image:1nze.gif|left|200px]]<br /><applet load="1nze" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1nze, resolution 1.95&Aring;" />
'''Crystal structure of PsbQ polypeptide of photosystem II from higher plants'''<br />


==Overview==
==Crystal structure of PsbQ polypeptide of photosystem II from higher plants==
The smallest extrinsic polypeptide of the water-oxidizing complex (PsbQ), was extracted and purified from spinach (Spinacia oleracea) photosystem II, (PSII) membranes. It was then crystallized in the presence of Zn(2+) and, its structure was determined by X-ray diffraction at 1.95-A resolution, using the multi-wavelength anomalous diffraction method, with the zinc as, the anomalous scatterer. The crystal structure shows that the core of the, protein is a four-helix bundle, whereas the amino-terminal portion, which, possibly interacts with the photosystem core, is not visible in the, crystal. The distribution of positive and negative charges on the protein, surface might explain the ability of PsbQ to increase the binding of Cl(-), and Ca(2+) and make them available to PSII.
<StructureSection load='1nze' size='340' side='right'caption='[[1nze]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1nze]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NZE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nze OCA], [https://pdbe.org/1nze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nze RCSB], [https://www.ebi.ac.uk/pdbsum/1nze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nze ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PSBQ_SPIOL PSBQ_SPIOL]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nz/1nze_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nze ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1NZE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NZE OCA].
*[[Photosystem II 3D structures|Photosystem II 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of the PsbQ protein of photosystem II from higher plants., Calderone V, Trabucco M, Vujicic A, Battistutta R, Giacometti GM, Andreucci F, Barbato R, Zanotti G, EMBO Rep. 2003 Sep;4(9):900-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12949587 12949587]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Spinacia oleracea]]
[[Category: Spinacia oleracea]]
[[Category: Andreucci, F.]]
[[Category: Andreucci F]]
[[Category: Barbato, R.]]
[[Category: Barbato R]]
[[Category: Battistutta, R.]]
[[Category: Battistutta R]]
[[Category: Calderone, V.]]
[[Category: Calderone V]]
[[Category: Giacometti, G.M.]]
[[Category: Giacometti GM]]
[[Category: Trabucco, M.]]
[[Category: Trabucco M]]
[[Category: Vujicic, A.]]
[[Category: Vujicic A]]
[[Category: Zanotti, G.]]
[[Category: Zanotti G]]
[[Category: ZN]]
[[Category: oee3]]
[[Category: oxygen-enhancer evolving complex]]
[[Category: photosystem ii]]
[[Category: psbq]]
[[Category: water oxidizing complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:41:11 2007''

Latest revision as of 08:00, 14 February 2024

Crystal structure of PsbQ polypeptide of photosystem II from higher plants

1nze, resolution 1.95Å

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