1obu: Difference between revisions

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New page: left|200px<br /><applet load="1obu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1obu, resolution 2.00Å" /> '''APOCRUSTACYANIN C1 C...
 
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[[Image:1obu.gif|left|200px]]<br /><applet load="1obu" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1obu, resolution 2.00&Aring;" />
'''APOCRUSTACYANIN C1 CRYSTALS GROWN IN SPACE AND EARTH USING VAPOUR DIFFUSION GEOMETRY'''<br />


==Overview==
==Apocrustacyanin C1 crystals grown in space and earth using vapour diffusion geometry==
Models of apocrustacyanin C(1) were refined against X-ray data recorded on, Bending Magnet 14 at the ESRF to resolutions of 1.85 and 2 A from a, space-grown and an earth-grown crystal, respectively, both using, vapour-diffusion crystal-growth geometry. The space crystals were grown in, the APCF on the NASA Space Shuttle. The microgravity crystal growth showed, a cyclic nature attributed to Marangoni convection, thus reducing the, benefits of the microgravity environment, as reported previously [Chayen, et al. (1996), Q. Rev. Biophys. 29, 227-278]. A subsequent mosaicity, evaluation, also reported previously, showed only a partial improvement in, the space-grown crystals over the earth-grown crystals [Snell et al., (1997), Acta Cryst. D53, 231-239], contrary to the case for lysozyme, crystals grown in space with liquid-liquid diffusion, i.e. without any, major motion during growth [Snell et al. (1995), Acta Cryst. D52, 1099-1102]. In this paper, apocrustacyanin C(1) electron-density maps from, the two refined models are now compared. It is concluded that the, electron-density maps of the protein and the bound waters are found to be, better overall for the structures of apocrustacyanin C(1) studied from the, space-grown crystal compared with those from the earth-grown crystal, even, though both crystals were grown using vapour-diffusion crystal-growth, geometry. The improved residues are on the surface of the protein, with, two involved in or nearby crystal lattice-forming interactions, thus, linking an improved crystal-growth mechanism to the molecular level. The, structural comparison procedures developed should themselves be valuable, for evaluating crystal-growth procedures in the future.
<StructureSection load='1obu' size='340' side='right'caption='[[1obu]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1obu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homarus_gammarus Homarus gammarus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OBU FirstGlance]. <br>
1OBU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homarus_gammarus Homarus gammarus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OBU OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1obu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obu OCA], [https://pdbe.org/1obu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1obu RCSB], [https://www.ebi.ac.uk/pdbsum/1obu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1obu ProSAT]</span></td></tr>
==Reference==
</table>
Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons., Habash J, Boggon TJ, Raftery J, Chayen NE, Zagalsky PF, Helliwell JR, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1117-23. Epub 2003, Jun 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12832753 12832753]
== Function ==
[https://www.uniprot.org/uniprot/CRC1_HOMGA CRC1_HOMGA] Binds the carotenoid astaxanthin (AXT) which provides the blue coloration to the carapace of the lobster.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/1obu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1obu ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Homarus gammarus]]
[[Category: Homarus gammarus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Boggon, T.J.]]
[[Category: Boggon TJ]]
[[Category: Chayen, N.E.]]
[[Category: Chayen NE]]
[[Category: Habash, J.]]
[[Category: Habash J]]
[[Category: Helliwell, J.R.]]
[[Category: Helliwell JR]]
[[Category: Raftery, J.]]
[[Category: Raftery J]]
[[Category: Zagalsky, P.F.]]
[[Category: Zagalsky PF]]
[[Category: antiparallel beta-strands]]
[[Category: lipocalin]]
[[Category: pigment]]
[[Category: transport protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:54:15 2007''

Latest revision as of 05:46, 17 April 2024

Apocrustacyanin C1 crystals grown in space and earth using vapour diffusion geometry

1obu, resolution 2.00Å

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