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New page: left|200px<br /><applet load="1oms" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oms, resolution 2.30Å" /> '''Structure determinat...
 
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[[Image:1oms.gif|left|200px]]<br /><applet load="1oms" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1oms, resolution 2.30&Aring;" />
'''Structure determination by MAD: E.coli Trigger Factor binding at the ribosomal exit tunnel.'''<br />


==Overview==
==Structure determination by MAD: E.coli Trigger Factor binding at the ribosomal exit tunnel.==
The exit tunnel region of the ribosome is well established as a focal, point for interaction between the components that guide the fate of, nascent polypeptides. One of these, the chaperone trigger factor (TF), associates with the 50S ribosomal subunit through its N-terminal domain., Targeting of TF to ribosomes is crucial to achieve its remarkable, efficiency in protein folding. A similar tight coupling to translation is, found in signal recognition particle (SRP)-dependent protein, translocation. Here, we report crystal structures of the E. coli TF, ribosome binding domain. TF is structurally related to the Hsp33 chaperone, but has a prominent ribosome anchor located as a tip of the molecule. This, tip includes the previously established unique TF signature motif., Comparison reveals that this feature is not found in SRP structures. We, identify a conserved helical kink as a hallmark of the TF structure that, is most likely critical to ensure ribosome association.
<StructureSection load='1oms' size='340' side='right'caption='[[1oms]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1oms]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OMS FirstGlance]. <br>
1OMS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, PG4, SO2 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OMS OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO2:SULFUR+DIOXIDE'>SO2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oms OCA], [https://pdbe.org/1oms PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oms RCSB], [https://www.ebi.ac.uk/pdbsum/1oms PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oms ProSAT]</span></td></tr>
Chaperone binding at the ribosomal exit tunnel., Kristensen O, Gajhede M, Structure. 2003 Dec;11(12):1547-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14656439 14656439]
</table>
== Function ==
[https://www.uniprot.org/uniprot/TIG_ECOLI TIG_ECOLI] Involved in protein export. Acts as a chaperone by maintaining the newly synthesized secretory and non-secretory proteins in an open conformation. Binds to nascent polypeptide chains via ribosomal protein L23 (PubMed:12226666). Functions as a peptidyl-prolyl cis-trans isomerase in vitro, this activity is dispensible in vivo for chaperone activity.<ref>PMID:8633085</ref> <ref>PMID:8521806</ref> <ref>PMID:14726952</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/om/1oms_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oms ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Peptidylprolyl isomerase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Gajhede M]]
[[Category: Gajhede, M.]]
[[Category: Kristensen O]]
[[Category: Kristensen, O.]]
[[Category: GOL]]
[[Category: PG4]]
[[Category: SO2]]
[[Category: SO4]]
[[Category: alpha-beta structure]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:00:49 2007''
Retrieved from "https://proteopedia.org/1oms"