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New page: left|200px<br /><applet load="1ops" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ops, resolution 2.00Å" /> '''ICE-BINDING SURFACE ...
 
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[[Image:1ops.jpg|left|200px]]<br /><applet load="1ops" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ops, resolution 2.00&Aring;" />
'''ICE-BINDING SURFACE ON A TYPE III ANTIFREEZE PROTEIN FROM OCEAN POUT'''<br />


==Overview==
==ICE-BINDING SURFACE ON A TYPE III ANTIFREEZE PROTEIN FROM OCEAN POUT==
Antifreeze proteins (AFPs) adsorb to surfaces of growing ice crystals, thereby arresting their growth. The prevailing hypothesis explains the, nature of adsorption in terms of a match between the hydrophilic side, chains on the AFP's ice-binding surface (IBS) and the water molecules on, the ice surface. The number and spatial arrangement of hydrogen bonds thus, formed have been proposed to account, respectively, for the binding, affinity and specificity. The crystal structure of a type III AFP from, ocean pout (isoform HPLC-3) has been determined to 2.0-A resolution. The, structure reveals an internal dyad motif formed by two 19-residue, loop-shaped elements. Based on of the flatness observed on the type I, alpha-helical AFP's IBS, an automated algorithm was developed to analyze, the surface planarity of the globular type III AFP and was used to, identify the IBS on this protein. The surface with the highest flatness, score is formed by one loop of the dyad motif and is identical to the IBS, deduced from earlier mutagenesis studies. Interestingly, 67% of this, surface contains nonpolar solvent-accessible surface area. The success of, our approach to identifying the IBS on an AFP, without considering the, presence of polar side chains, indicates that flatness is the first, approximation of an IBS. We further propose that the specificity of, interactions between an IBS and a particular ice-crystallographic plane, arises from surface complementarity.
<StructureSection load='1ops' size='340' side='right'caption='[[1ops]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ops]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OPS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ops FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ops OCA], [https://pdbe.org/1ops PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ops RCSB], [https://www.ebi.ac.uk/pdbsum/1ops PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ops ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ANP1_ZOAAM ANP1_ZOAAM] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/1ops_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ops ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1OPS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Macrozoarces_americanus Macrozoarces americanus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OPS OCA].
*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Identification of the ice-binding surface on a type III antifreeze protein with a "flatness function" algorithm., Yang DS, Hon WC, Bubanko S, Xue Y, Seetharaman J, Hew CL, Sicheri F, Biophys J. 1998 May;74(5):2142-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9591641 9591641]
[[Category: Large Structures]]
[[Category: Macrozoarces americanus]]
[[Category: Zoarces americanus]]
[[Category: Single protein]]
[[Category: Bubanko S]]
[[Category: Bubanko, S.]]
[[Category: Hew CL]]
[[Category: Hew, C.L.]]
[[Category: Hon W-C]]
[[Category: Hon, W.C.]]
[[Category: Seetharaman J]]
[[Category: Seetharaman, J.]]
[[Category: Sicheri F]]
[[Category: Sicheri, F.]]
[[Category: Xue Y]]
[[Category: Xue, Y.]]
[[Category: Yang DSC]]
[[Category: Yang, D.S.C.]]
[[Category: antifreeze protein]]
[[Category: glycoprotein]]
[[Category: ice crystal growth inhibition]]
[[Category: pretzel fold]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:05:37 2007''
Retrieved from "https://proteopedia.org/1ops"