1orc: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="1orc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1orc, resolution 1.54Å" /> '''CRO REPRESSOR INSERT...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1orc.gif|left|200px]]<br /><applet load="1orc" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1orc, resolution 1.54&Aring;" />
'''CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]'''<br />


==Overview==
==CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]==
A rationally designed, genetically engineered, monomeric form of the Cro, protein from bacteriophage lambda has been crystallized and its structure, determined by isomorphous replacement and refined to a resolution of 1.54, A. The structure confirms the rationale of the design but, at the same, time, reveals 1-2 A shifts throughout the monomer structure relative to, the previously determined structure of the dimeric wild-type protein., These changes include a 1.6 A main-chain shift in part of the beta-sheet, region of the molecule relative to the alpha-helical region and a 1.1 A, shift of a buried phenylalanine within the core as well as a correlated, 2.2 A shift in a solvent-exposed beta-hairpin. The conformational, adjustments appear to reflect an inherent flexibility of the protein that, is associated with its DNA-binding function.
<StructureSection load='1orc' size='340' side='right'caption='[[1orc]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1orc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ORC FirstGlance]. <br>
1ORC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ORC OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1orc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1orc OCA], [https://pdbe.org/1orc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1orc RCSB], [https://www.ebi.ac.uk/pdbsum/1orc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1orc ProSAT]</span></td></tr>
==Reference==
</table>
High-resolution structure of an engineered Cro monomer shows changes in conformation relative to the native dimer., Albright RA, Mossing MC, Matthews BW, Biochemistry. 1996 Jan 23;35(3):735-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8547253 8547253]
== Function ==
[[Category: Enterobacteria phage lambda]]
[https://www.uniprot.org/uniprot/RCRO_LAMBD RCRO_LAMBD] Cro represses genes normally expressed in early phage development and is necessary for the late stage of lytic growth. It does this by binding to the OL and OR operators regions normally used by the repressor protein for lysogenic maintenance.
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Albright, R.A.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Matthews, B.W.]]
Check<jmol>
[[Category: Mossing, M.C.]]
  <jmolCheckbox>
[[Category: gene regulating protein]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/or/1orc_consurf.spt"</scriptWhenChecked>
 
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:07:33 2007''
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1orc ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia virus Lambda]]
[[Category: Large Structures]]
[[Category: Albright RA]]
[[Category: Matthews BW]]
[[Category: Mossing MC]]

Latest revision as of 08:02, 14 February 2024

CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]

1orc, resolution 1.54Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1orc&oldid=4052877"