1tne: Difference between revisions

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{{Seed}}
[[Image:1tne.png|left|200px]]


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==NMR STUDY OF Z-DNA AT PHYSIOLOGICAL SALT CONDITIONS, MINIMIZED AVERAGE STRUCTURE==
The line below this paragraph, containing "STRUCTURE_1tne", creates the "Structure Box" on the page.
<StructureSection load='1tne' size='340' side='right'caption='[[1tne]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1tne]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TNE FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8MG:8-METHYL-2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>8MG</scene></td></tr>
{{STRUCTURE_1tne|  PDB=1tne  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tne OCA], [https://pdbe.org/1tne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tne RCSB], [https://www.ebi.ac.uk/pdbsum/1tne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tne ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Various oligonucleotides containing 8-methylguanine (m8G) have been synthesized and their structures and thermodynamic properties investigated. Introduction Of M8G into DNA sequences markedly stabilizes the Z conformation under low salt conditions. The hexamer d(CGC[M8G]CG)2 exhibits a CD spectrum characteristic of the Z conformation under physiological salt conditions. The NOE-restrained refinement unequivocally demonstrated that d(CGC[m8G]CG)2 adopts a Z structure with all guanines in the syn conformation. The refined NMR structure is very similar to the Z form crystal structure of d(CGCGCG)2, with a root mean square deviation of 0.6 between the two structures. The contribution of m8G to the stabilization of Z-DNA has been estimated from the mid-point NaCl concentrations for the B-Z transition of various m8G-containing oligomers. The presence of m8G in d(CGC[m8G]CG)2 stabilizes the Z conformation by at least deltaG = -0.8 kcal/mol relative to the unmodified hexamer. The Z conformation was further stabilized by increasing the number of m8Gs incorporated and destabilized by incorporating syn-A or syn-T, found respectively in the (A,T)-containing alternating and non-alternating pyrimidine-purine sequences. The results suggest that the chemically less reactive m8G base is a useful agent for studying molecular interactions of Z-DNA or other DNA structures that incorporate syn-G conformation.


===NMR STUDY OF Z-DNA AT PHYSIOLOGICAL SALT CONDITIONS, MINIMIZED AVERAGE STRUCTURE===
Synthesis, structure and thermodynamic properties of 8-methylguanine-containing oligonucleotides: Z-DNA under physiological salt conditions.,Sugiyama H, Kawai K, Matsunaga A, Fujimoto K, Saito I, Robinson H, Wang AH Nucleic Acids Res. 1996 Apr 1;24(7):1272-8. PMID:8614630<ref>PMID:8614630</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1tne" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_8614630}}, adds the Publication Abstract to the page
*[[Z-DNA|Z-DNA]]
(as it appears on PubMed at http://www.pubmed.gov), where 8614630 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_8614630}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TNE OCA].
[[Category: Fujimoto K]]
 
[[Category: Kawai K]]
==Reference==
[[Category: Matsunaga A]]
Synthesis, structure and thermodynamic properties of 8-methylguanine-containing oligonucleotides: Z-DNA under physiological salt conditions., Sugiyama H, Kawai K, Matsunaga A, Fujimoto K, Saito I, Robinson H, Wang AH, Nucleic Acids Res. 1996 Apr 1;24(7):1272-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8614630 8614630]
[[Category: Robinson H]]
[[Category: Fujimoto, K.]]
[[Category: Saito I]]
[[Category: Kawai, K.]]
[[Category: Sugiyama H]]
[[Category: Matsunaga, A.]]
[[Category: Wang AH-J]]
[[Category: Robinson, H.]]
[[Category: Saito, I.]]
[[Category: Sugiyama, H.]]
[[Category: Wang, A H.J.]]
[[Category: Z-dna]]
 
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