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New page: left|200px<br /><applet load="1osz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1osz, resolution 2.1Å" /> '''MHC CLASS I H-2KB HEA...
 
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[[Image:1osz.jpg|left|200px]]<br /><applet load="1osz" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1osz, resolution 2.1&Aring;" />
'''MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN'''<br />


==Overview==
==MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN==
The T lineage repertoire is shaped by T cell receptor (TCR)-dependent, positive and negative thymic selection processes. Using TCR-transgenic, (N15tg) beta2-microglobulin-deficient (beta2m-/-) RAG-2(-/-) H-2(b) mice, specific for the VSV8 (RGYVYQGL) octapeptide bound to Kb, we identified a, single weak agonist peptide variant V4L (L4) inducing phenotypic and, functional T cell maturation. The cognate VSV8 peptide, in contrast, triggers negative selection. The crystal structure of L4/Kb was determined, and refined to 2.1 A for comparison with the VSV8/Kb structure at similar, resolution. Aside from changes on the p4 side chain of L4 and the, resulting alteration of the exposed Kb Lys-66 side chain, these two, structures are essentially identical. Hence, a given TCR recognizes subtle, distinctions between highly related ligands, resulting in dramatically, different selection outcomes. Based on these finding and the recent, structural elucidation of the N15-VSV8/Kb complex, moreover, it appears, that the germ-line Valpha repertoire contributes in a significant way to, positive selection.
<StructureSection load='1osz' size='340' side='right'caption='[[1osz]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1osz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OSZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1osz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1osz OCA], [https://pdbe.org/1osz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1osz RCSB], [https://www.ebi.ac.uk/pdbsum/1osz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1osz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B2MG_MOUSE B2MG_MOUSE] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/os/1osz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1osz ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The T lineage repertoire is shaped by T cell receptor (TCR)-dependent positive and negative thymic selection processes. Using TCR-transgenic (N15tg) beta2-microglobulin-deficient (beta2m-/-) RAG-2(-/-) H-2(b) mice specific for the VSV8 (RGYVYQGL) octapeptide bound to Kb, we identified a single weak agonist peptide variant V4L (L4) inducing phenotypic and functional T cell maturation. The cognate VSV8 peptide, in contrast, triggers negative selection. The crystal structure of L4/Kb was determined and refined to 2.1 A for comparison with the VSV8/Kb structure at similar resolution. Aside from changes on the p4 side chain of L4 and the resulting alteration of the exposed Kb Lys-66 side chain, these two structures are essentially identical. Hence, a given TCR recognizes subtle distinctions between highly related ligands, resulting in dramatically different selection outcomes. Based on these finding and the recent structural elucidation of the N15-VSV8/Kb complex, moreover, it appears that the germ-line Valpha repertoire contributes in a significant way to positive selection.


==About this Structure==
Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands.,Ghendler Y, Teng MK, Liu JH, Witte T, Liu J, Kim KS, Kern P, Chang HC, Wang JH, Reinherz EL Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10061-6. PMID:9707600<ref>PMID:9707600</ref>
1OSZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OSZ OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands., Ghendler Y, Teng MK, Liu JH, Witte T, Liu J, Kim KS, Kern P, Chang HC, Wang JH, Reinherz EL, Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10061-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9707600 9707600]
</div>
<div class="pdbe-citations 1osz" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Chang H-C]]
[[Category: Chang, H.C.]]
[[Category: Ghendler Y]]
[[Category: Ghendler, Y.]]
[[Category: Kern P]]
[[Category: Kern, P.]]
[[Category: Kim KS]]
[[Category: Kim, K.S.]]
[[Category: Liu J]]
[[Category: Liu, J.]]
[[Category: Liu J-H]]
[[Category: Liu, J.H.]]
[[Category: Reinherz EL]]
[[Category: Reinherz, E.L.]]
[[Category: Teng M-K]]
[[Category: Teng, M.K.]]
[[Category: Wang J-H]]
[[Category: Wang, J.H.]]
[[Category: Witte T]]
[[Category: Witte, T.]]
[[Category: complex (mhc i/peptide)]]
[[Category: mhc/peptide complex]]
[[Category: thymic selection]]
[[Category: transmembrane protein]]
 
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