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New page: left|200px<br /><applet load="1ox7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ox7, resolution 1.43Å" /> '''Crystal structure of...
 
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[[Image:1ox7.gif|left|200px]]<br /><applet load="1ox7" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ox7, resolution 1.43&Aring;" />
'''Crystal structure of yeast cytosine deaminase apo-enzyme: inorganic zinc bound'''<br />


==Overview==
==Crystal structure of yeast cytosine deaminase apo-enzyme: inorganic zinc bound==
Cytosine deaminase (CD) catalyzes the deamination of cytosine and is only, present in prokaryotes and fungi, where it is a member of the pyrimidine, salvage pathway. The enzyme is of interest both for antimicrobial drug, design and gene therapy applications against tumors. The structure of, Saccharomyces cerevisiae CD has been determined in the presence and, absence of a mechanism-based inhibitor, at 1.14 and 1.43 A resolution, respectively. The enzyme forms an alpha/beta fold similar to bacterial, cytidine deaminase, but with no similarity to the alpha/beta barrel fold, used by bacterial cytosine deaminase or mammalian adenosine deaminase. The, structures observed for bacterial, fungal, and mammalian nucleic acid, deaminases represent an example of the parallel evolution of two unique, protein folds to carry out the same reaction on a diverse array of, substrates.
<StructureSection load='1ox7' size='340' side='right'caption='[[1ox7]], [[Resolution|resolution]] 1.43&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ox7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OX7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.43&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ox7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ox7 OCA], [https://pdbe.org/1ox7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ox7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ox7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ox7 ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/1ox7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ox7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cytosine deaminase (CD) catalyzes the deamination of cytosine and is only present in prokaryotes and fungi, where it is a member of the pyrimidine salvage pathway. The enzyme is of interest both for antimicrobial drug design and gene therapy applications against tumors. The structure of Saccharomyces cerevisiae CD has been determined in the presence and absence of a mechanism-based inhibitor, at 1.14 and 1.43 A resolution, respectively. The enzyme forms an alpha/beta fold similar to bacterial cytidine deaminase, but with no similarity to the alpha/beta barrel fold used by bacterial cytosine deaminase or mammalian adenosine deaminase. The structures observed for bacterial, fungal, and mammalian nucleic acid deaminases represent an example of the parallel evolution of two unique protein folds to carry out the same reaction on a diverse array of substrates.


==About this Structure==
The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy.,Ireton GC, Black ME, Stoddard BL Structure. 2003 Aug;11(8):961-72. PMID:12906827<ref>PMID:12906827</ref>
1OX7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytosine_deaminase Cytosine deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.1 3.5.4.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OX7 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy., Ireton GC, Black ME, Stoddard BL, Structure. 2003 Aug;11(8):961-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12906827 12906827]
</div>
[[Category: Cytosine deaminase]]
<div class="pdbe-citations 1ox7" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Deaminase 3D structures|Deaminase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Black ME]]
[[Category: Black, M.E.]]
[[Category: Ireton GC]]
[[Category: Ireton, G.C.]]
[[Category: Stoddard BL]]
[[Category: Stoddard, B.L.]]
[[Category: CA]]
[[Category: ZN]]
[[Category: aminohydrolase]]
 
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