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New page: left|200px<br /><applet load="1ozb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ozb, resolution 2.80Å" /> '''Crystal Structure of...
 
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[[Image:1ozb.gif|left|200px]]<br /><applet load="1ozb" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ozb, resolution 2.80&Aring;" />
'''Crystal Structure of SecB complexed with SecA C-terminus'''<br />


==Overview==
==Crystal Structure of SecB complexed with SecA C-terminus==
SecB is a bacterial chaperone involved in directing pre-protein to the, translocation pathway by its specific interaction with the peripheral, membrane ATPase SecA. The SecB-binding site on SecA is located at its C, terminus and consists of a stretch of highly conserved residues. The, crystal structure of SecB in complex with the C-terminal 27 amino acids of, SecA from Haemophilus influenzae shows that the SecA peptide is structured, as a CCCH zinc-binding motif. One SecB tetramer is bound by two SecA, peptides, and the interface involves primarily salt bridges and hydrogen, bonding interactions. The structure explains the importance of the, zinc-binding motif and conserved residues at the C terminus of SecA in its, high-affinity binding with SecB. It also suggests a model of SecB-SecA, interaction and its implication for the mechanism of pre-protein transfer, in bacterial protein translocation.
<StructureSection load='1ozb' size='340' side='right'caption='[[1ozb]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ozb]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OZB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozb OCA], [https://pdbe.org/1ozb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ozb RCSB], [https://www.ebi.ac.uk/pdbsum/1ozb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SECB_HAEIN SECB_HAEIN] One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1ozb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ozb ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1OZB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OZB OCA].
*[[Preprotein translocase 3D structures|Preprotein translocase 3D structures]]
 
*[[SecA|SecA]]
==Reference==
__TOC__
Structural determinants of SecB recognition by SecA in bacterial protein translocation., Zhou J, Xu Z, Nat Struct Biol. 2003 Nov;10(11):942-7. Epub 2003 Sep 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14517549 14517549]
</StructureSection>
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Xu, Z.]]
[[Category: Xu Z]]
[[Category: Zhou, J.]]
[[Category: Zhou J]]
[[Category: ZN]]
[[Category: protein-protein complex]]
[[Category: zinc binding motif]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:19:09 2007''

Latest revision as of 08:04, 14 February 2024

Crystal Structure of SecB complexed with SecA C-terminus

1ozb, resolution 2.80Å

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