1p7o: Difference between revisions

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New page: left|200px<br /><applet load="1p7o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p7o, resolution 2.30Å" /> '''Crystal structure of...
 
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[[Image:1p7o.jpg|left|200px]]<br /><applet load="1p7o" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1p7o, resolution 2.30&Aring;" />
'''Crystal structure of phospholipase A2 (MIPLA4) from Micropechis ikaheka'''<br />


==Overview==
==Crystal structure of phospholipase A2 (MIPLA4) from Micropechis ikaheka==
Comparison of the crystal structures of three Micropechis ikaheka, phospholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit, different levels of pharmacological effects) shows that their C-terminus, (residues 110-124) is the most variable. M-Type receptor binding affinity, of the isoenzymes has also been investigated and MiPLA4 binds to the, rabbit M-type receptor with high affinity. Examination of surface charges, of the isoenzymes reveals a trend of increase in positive charges with, potency. The isoenzymes are shown to oligomerize in a, concentration-dependent manner in a semi-denaturing gel. The C-termini of, the medium (MiPLA4) and highly potent (MiPLA2) isoenzyme molecules cluster, together, forming a highly exposed area. A BLAST search using the sequence, of the most potent MiPLA2 results in high similarity to Staphylococcus, aureus clotting factor A and cadherin 11. This might explain the, myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s.
<StructureSection load='1p7o' size='340' side='right'caption='[[1p7o]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1p7o]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Micropechis_ikaheca Micropechis ikaheca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P7O FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p7o OCA], [https://pdbe.org/1p7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p7o RCSB], [https://www.ebi.ac.uk/pdbsum/1p7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p7o ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PA2A2_TROCA PA2A2_TROCA] PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p7/1p7o_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p7o ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1P7O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Micropechis_ikaheka Micropechis ikaheka]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P7O OCA].
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure and function comparison of Micropechis ikaheka snake venom phospholipase A2 isoenzymes., Lok SM, Gao R, Rouault M, Lambeau G, Gopalakrishnakone P, Swaminathan K, FEBS J. 2005 Mar;272(5):1211-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15720395 15720395]
[[Category: Large Structures]]
[[Category: Micropechis ikaheka]]
[[Category: Micropechis ikaheca]]
[[Category: Phospholipase A(2)]]
[[Category: Lok SM]]
[[Category: Protein complex]]
[[Category: Swaminathan K]]
[[Category: Lok, S.M.]]
[[Category: Swaminathan, K.]]
[[Category: micropechis ikaheka]]
[[Category: pancreatic loop]]
[[Category: phospholipase a2]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:33:30 2007''

Latest revision as of 06:10, 3 April 2024

Crystal structure of phospholipase A2 (MIPLA4) from Micropechis ikaheka

1p7o, resolution 2.30Å

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