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New page: left|200px<br /><applet load="1pdi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pdi" /> '''Fitting of the C-terminal part of the short ...
 
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[[Image:1pdi.gif|left|200px]]<br /><applet load="1pdi" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1pdi" />
'''Fitting of the C-terminal part of the short tail fibers into the cryo-EM reconstruction of T4 baseplate'''<br />


==Overview==
==Fitting of the C-terminal part of the short tail fibers into the cryo-EM reconstruction of T4 baseplate==
The baseplate of bacteriophage T4 is a multiprotein molecular machine that, controls host cell recognition, attachment, tail sheath contraction and, viral DNA ejection. We report here the three-dimensional structure of the, baseplate-tail tube complex determined to a resolution of 12 A by, cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like, structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the, center of the dome is a needle-like structure that was previously, identified as a cell puncturing device. We have identified the locations, of six proteins with known atomic structures, and established the position, and shape of several other baseplate proteins. The baseplate structure, suggests a mechanism of baseplate triggering and structural transition, during the initial stages of T4 infection.
<SX load='1pdi' size='340' side='right' viewer='molstar' caption='[[1pdi]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1pdi]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PDI FirstGlance]. <br>
1PDI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PDI OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pdi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pdi OCA], [https://pdbe.org/1pdi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pdi RCSB], [https://www.ebi.ac.uk/pdbsum/1pdi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pdi ProSAT]</span></td></tr>
==Reference==
</table>
Three-dimensional structure of bacteriophage T4 baseplate., Kostyuchenko VA, Leiman PG, Chipman PR, Kanamaru S, van Raaij MJ, Arisaka F, Mesyanzhinov VV, Rossmann MG, Nat Struct Biol. 2003 Sep;10(9):688-93. Epub 2003 Aug 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12923574 12923574]
== Function ==
[[Category: Bacteriophage t4]]
[https://www.uniprot.org/uniprot/FIB12_BPT4 FIB12_BPT4] Structural component of the short tail fiber. Adhesion protein that binds irreversibly to the lipopolysaccharides component (LPS) on the cell surface of Escherichia coli B strains during virus attachment. After at least three long tail fibers have bound, short tail fibers extend and bind irreversibly to the core region of the host cell LPS.<ref>PMID:12837775</ref>
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Arisaka, F.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Chipman, P.R.]]
Check<jmol>
[[Category: Kanamaru, S.]]
  <jmolCheckbox>
[[Category: Kostyuchenko, V.A.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pd/1pdi_consurf.spt"</scriptWhenChecked>
[[Category: Leiman, P.G.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Mesyanzhinov, V.V.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Raaij, M.J.van.]]
  </jmolCheckbox>
[[Category: Rossmann, M.G.]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pdi ConSurf].
[[Category: structural protein]]
<div style="clear:both"></div>
 
== References ==
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:42:41 2007''
<references/>
__TOC__
</SX>
[[Category: Escherichia virus T4]]
[[Category: Large Structures]]
[[Category: Arisaka F]]
[[Category: Chipman PR]]
[[Category: Kanamaru S]]
[[Category: Kostyuchenko VA]]
[[Category: Leiman PG]]
[[Category: Mesyanzhinov VV]]
[[Category: Rossmann MG]]
[[Category: Van Raaij MJ]]

Latest revision as of 08:07, 14 February 2024

Fitting of the C-terminal part of the short tail fibers into the cryo-EM reconstruction of T4 baseplate

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