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New page: left|200px<br /><applet load="1pdl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pdl" /> '''Fitting of gp5 in the cryoEM reconstruction ...
 
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[[Image:1pdl.jpg|left|200px]]<br /><applet load="1pdl" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1pdl" />
'''Fitting of gp5 in the cryoEM reconstruction of the bacteriophage T4 baseplate'''<br />


==Overview==
==Fitting of gp5 in the cryoEM reconstruction of the bacteriophage T4 baseplate==
The baseplate of bacteriophage T4 is a multiprotein molecular machine that, controls host cell recognition, attachment, tail sheath contraction and, viral DNA ejection. We report here the three-dimensional structure of the, baseplate-tail tube complex determined to a resolution of 12 A by, cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like, structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the, center of the dome is a needle-like structure that was previously, identified as a cell puncturing device. We have identified the locations, of six proteins with known atomic structures, and established the position, and shape of several other baseplate proteins. The baseplate structure, suggests a mechanism of baseplate triggering and structural transition, during the initial stages of T4 infection.
<SX load='1pdl' size='340' side='right' viewer='molstar' caption='[[1pdl]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1pdl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PDL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pdl OCA], [https://pdbe.org/1pdl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pdl RCSB], [https://www.ebi.ac.uk/pdbsum/1pdl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pdl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NEEDL_BPT4 NEEDL_BPT4] Tail-associated lysozyme of the baseplate hub that is essential for localized hydrolysis of bacterial cell wall necessary for viral DNA injection. The needle-like gp5 protein punctures the outer cell membrane and then digests the peptidoglycan cell wall in the periplasmic space. Involved in the tail assembly.<ref>PMID:12837775</ref> <ref>PMID:21129200</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pd/1pdl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pdl ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1PDL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PDL OCA].
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
 
== References ==
==Reference==
<references/>
Three-dimensional structure of bacteriophage T4 baseplate., Kostyuchenko VA, Leiman PG, Chipman PR, Kanamaru S, van Raaij MJ, Arisaka F, Mesyanzhinov VV, Rossmann MG, Nat Struct Biol. 2003 Sep;10(9):688-93. Epub 2003 Aug 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12923574 12923574]
__TOC__
[[Category: Bacteriophage t4]]
</SX>
[[Category: Lysozyme]]
[[Category: Escherichia virus T4]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Arisaka, F.]]
[[Category: Arisaka F]]
[[Category: Chipman, P.R.]]
[[Category: Chipman PR]]
[[Category: Kanamaru, S.]]
[[Category: Kanamaru S]]
[[Category: Kostyuchenko, V.A.]]
[[Category: Kostyuchenko VA]]
[[Category: Leiman, P.G.]]
[[Category: Leiman PG]]
[[Category: Mesyanzhinov, V.V.]]
[[Category: Mesyanzhinov VV]]
[[Category: Raaij, M.J.van.]]
[[Category: Rossmann MG]]
[[Category: Rossmann, M.G.]]
[[Category: Van Raaij MJ]]
[[Category: hydrolase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:42:56 2007''

Latest revision as of 08:07, 14 February 2024

Fitting of gp5 in the cryoEM reconstruction of the bacteriophage T4 baseplate

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