2chv: Difference between revisions

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{{Seed}}
[[Image:2chv.png|left|200px]]


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==Replication Factor C ADPNP complex==
The line below this paragraph, containing "STRUCTURE_2chv", creates the "Structure Box" on the page.
<StructureSection load='2chv' size='340' side='right'caption='[[2chv]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2chv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CHV FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2chv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2chv OCA], [https://pdbe.org/2chv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2chv RCSB], [https://www.ebi.ac.uk/pdbsum/2chv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2chv ProSAT]</span></td></tr>
{{STRUCTURE_2chv|  PDB=2chv  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/RFCS_ARCFU RFCS_ARCFU] Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.<ref>PMID:12384579</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/2chv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2chv ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity, a process that normally only requires binding of nucleotide. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. However, this symmetry is broken when the nucleotide is bound to the complex. A conformational change associated with nucleotide binding may relate to the opening of PCNA rings by RFC during the loading reaction. The structures also reveal the importance of the N-terminal helix of each subunit at the ATP-binding site. Analysis of mutant protein complexes containing subunits lacking this N-terminal helix reveals key distinct regulatory roles during clamp loading that are different for the large and small subunits in the RFC complex.


===REPLICATION FACTOR C ADPNP COMPLEX===
Communication between subunits within an archaeal clamp-loader complex.,Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB EMBO J. 2006 May 17;25(10):2209-18. Epub 2006 Apr 20. PMID:16628222<ref>PMID:16628222</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_16628222}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2chv" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 16628222 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_16628222}}
__TOC__
 
</StructureSection>
==About this Structure==
2CHV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CHV OCA].
 
==Reference==
Communication between subunits within an archaeal clamp-loader complex., Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB, EMBO J. 2006 May 17;25(10):2209-18. Epub 2006 Apr 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16628222 16628222]
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cook, N.]]
[[Category: Cook N]]
[[Category: Hall, D R.]]
[[Category: Hall DR]]
[[Category: Seybert, A.]]
[[Category: Seybert A]]
[[Category: Singleton, M R.]]
[[Category: Singleton MR]]
[[Category: Wigley, D B.]]
[[Category: Wigley DB]]
[[Category: Aaa+ atpase]]
[[Category: Atp-binding]]
[[Category: Clamp loader]]
[[Category: Dna replication]]
[[Category: Dna-binding protein]]
[[Category: Nucleotide-binding]]
 
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Retrieved from "https://proteopedia.org/2chv"