2evn: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{Seed}}
[[Image:2evn.png|left|200px]]


<!--
==NMR solution structures of At1g77540==
The line below this paragraph, containing "STRUCTURE_2evn", creates the "Structure Box" on the page.
<StructureSection load='2evn' size='340' side='right'caption='[[2evn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2evn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1xo4 1xo4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EVN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EVN FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2evn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2evn OCA], [https://pdbe.org/2evn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2evn RCSB], [https://www.ebi.ac.uk/pdbsum/2evn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2evn ProSAT], [https://www.topsan.org/Proteins/CESG/2evn TOPSAN]</span></td></tr>
-->
</table>
{{STRUCTURE_2evn|  PDB=2evn  |  SCENE=  }}
== Function ==
[[https://www.uniprot.org/uniprot/Y1754_ARATH Y1754_ARATH]] Possesses in vitro histone acetyltransferase activity with histones H3 and H4.<ref>PMID:17128971</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ev/2evn_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2evn ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We describe X-ray crystal and NMR solution structures of the protein coded for by Arabidopsis thaliana gene At1g77540.1 (At1g77540). The crystal structure was determined to 1.15 A with an R factor of 14.9% (Rfree = 17.0%) by multiple-wavelength anomalous diffraction using sodium bromide derivatized crystals. The ensemble of NMR conformers was determined with protein samples labeled with 15N and 13C + 15N. The X-ray structure and NMR ensemble were closely similar with rmsd 1.4 A for residues 8-93. At1g77540 was found to adopt a fold similar to that of GCN5-related N-acetyltransferases. Enzymatic activity assays established that At1g77540 possesses weak acetyltransferase activity against histones H3 and H4. Chemical shift perturbations observed in 15N-HSQC spectra upon the addition of CoA indicated that the cofactor binds and identified its binding site. The molecular details of this interaction were further elucidated by solving the X-ray structure of the At1g77540-CoA complex. This work establishes that the domain family COG2388 represents a novel class of acetyltransferase and provides insight into possible mechanistic roles of the conserved Cys76 and His41 residues of this family.


===NMR solution structures of At1g77540===
Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family.,Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:17128971<ref>PMID:17128971</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<!--
</div>
The line below this paragraph, {{ABSTRACT_PUBMED_17128971}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2evn" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 17128971 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_17128971}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Arath]]
2EVN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1xo4 1xo4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EVN OCA].
[[Category: Large Structures]]
 
[[Category: Structural genomic]]
==Reference==
[[Category: Markley, J L]]
Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17128971 17128971]
[[Category: Min, M S]]
[[Category: Arabidopsis thaliana]]
[[Category: Singh, S]]
[[Category: Single protein]]
[[Category: Tonelli, M]]
[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
[[Category: Tyler, R C]]
[[Category: Markley, J L.]]
[[Category: PSI, Protein structure initiative]]
[[Category: Min, M S.]]
[[Category: Unknown function]]
[[Category: Singh, S.]]
[[Category: Tonelli, M.]]
[[Category: Tyler, R C.]]
[[Category: Protein structure initiative]]
[[Category: Psi structural genomics cesg at1g77540 center for eukaryotic structural genomic]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:50:42 2008''

Latest revision as of 15:58, 22 December 2021

NMR solution structures of At1g77540

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2evn&oldid=3493649"