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New page: left|200px<br /><applet load="1pfi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pfi, resolution 3.0Å" /> '''PF1 VIRUS STRUCTURE: ...
 
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[[Image:1pfi.gif|left|200px]]<br /><applet load="1pfi" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1pfi, resolution 3.0&Aring;" />
'''PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES'''<br />


==Overview==
==PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES==
The helical path of the DNA in filamentous bacteriophage Pf1 was deduced, from different kinds of existing structural information, including results, from x-ray fiber diffraction. The DNA has the same pitch, 16 angstroms, as, the surrounding helix of protein subunits; the rise and rotation per, nucleotides are 6.1 angstroms and 132 degrees, respectively; and the, phosphates are 2.5 angstroms from the axis. The DNA in Pf1 is, therefore, the most extended and twisted DNA structure known. On the basis of the DNA, structure and extensive additional information about the protein, a model, of the virion is proposed. In the model, the DNA bases reach out, into the, protein, and the lysine and arginine side chains reach in, between the DNA, bases, to stabilize the paraxial phosphate charges; the conformation of, the protein subunit is a combination of alpha and 3(10) helices.
<StructureSection load='1pfi' size='340' side='right'caption='[[1pfi]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1pfi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_Pf1 Pseudomonas virus Pf1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PFI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Fiber diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfi OCA], [https://pdbe.org/1pfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pfi RCSB], [https://www.ebi.ac.uk/pdbsum/1pfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pfi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAPSD_BPPF1 CAPSD_BPPF1] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).


==About this Structure==
==See Also==
1PFI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1] with C as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PFI OCA].
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Pf1 virus structure: helical coat protein and DNA with paraxial phosphates., Liu DJ, Day LA, Science. 1994 Jul 29;265(5172):671-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8036516 8036516]
[[Category: Large Structures]]
[[Category: Pseudomonas phage pf1]]
[[Category: Pseudomonas virus Pf1]]
[[Category: Single protein]]
[[Category: Day LA]]
[[Category: Day, L.A.]]
[[Category: Liu DJ]]
[[Category: Liu, D.J.]]
[[Category: C]]
[[Category: complex(viral coat protein/dna)]]
[[Category: helical virus]]
 
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