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New page: left|200px<br /><applet load="1pgx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pgx, resolution 1.66Å" /> '''THE 1.66 ANGSTROMS X...
 
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[[Image:1pgx.gif|left|200px]]<br /><applet load="1pgx" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1pgx, resolution 1.66&Aring;" />
'''THE 1.66 ANGSTROMS X-RAY STRUCTURE OF THE B2 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON TO THE NMR STRUCTURE OF THE B1 DOMAIN'''<br />


==Overview==
==THE 1.66 ANGSTROMS X-RAY STRUCTURE OF THE B2 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON TO THE NMR STRUCTURE OF THE B1 DOMAIN==
The structure of the B2 immunoglobulin-binding domain of streptococcal, protein G has been determined at 1.67-A resolution using a combination of, single isomorphous replacement (SIR) phasing and manual fitting of the, coordinates of the NMR structure of B1 domain of streptococcal protein G, [Gronenborn, A. M., et al. (1991) Science 253, 657-661]. The final R value, was 0.191 for data between 8.0 and 1.67 A. The structure described here, has 13 residues preceding the 57-residue Ig-binding domain and 13, additional residues following it, for a total of 83 residues. The, 57-residue binding domain is well-determined in the structure, having an, average B factor of 18.0. Only residues 8-77 could be located in the, electron density maps, with the ends of the structure fading into, disorder. Like the B1 domain, the B2 domain consists of four beta-strands, and a single helix lying diagonally across the beta-sheet, with a -1, +3, chi, -1 topology. This small structure is extensively hydrogen-bonded and, has a relatively large hydrophobic core. These structural observations may, account for the exceptional stability of protein G. A comparison of the B2, domain X-ray structure and the B1 domain NMR structure showed minor, differences in the turn between strands and two and a slight displacement, of the helix relative to the sheet. Hydrogen bonds between, crystallographically related molecules account for most of these, differences.
<StructureSection load='1pgx' size='340' side='right'caption='[[1pgx]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1pgx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus Streptococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PGX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PGX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pgx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pgx OCA], [https://pdbe.org/1pgx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pgx RCSB], [https://www.ebi.ac.uk/pdbsum/1pgx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pgx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPG1_STRSG SPG1_STRSG] Binds to the constant Fc region of IgG with high affinity.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pg/1pgx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pgx ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1PGX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcaceae Streptococcaceae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PGX OCA].
*[[Protein G|Protein G]]
 
__TOC__
==Reference==
</StructureSection>
1.67-A X-ray structure of the B2 immunoglobulin-binding domain of streptococcal protein G and comparison to the NMR structure of the B1 domain., Achari A, Hale SP, Howard AJ, Clore GM, Gronenborn AM, Hardman KD, Whitlow M, Biochemistry. 1992 Nov 3;31(43):10449-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1420164 1420164]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Streptococcus]]
[[Category: Streptococcaceae]]
[[Category: Achari A]]
[[Category: Achari, A.]]
[[Category: Howard AJ]]
[[Category: Howard, A.J.]]
[[Category: Whitlow M]]
[[Category: Whitlow, M.]]
[[Category: immunoglobulin binding protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:47:54 2007''

Latest revision as of 08:07, 14 February 2024

THE 1.66 ANGSTROMS X-RAY STRUCTURE OF THE B2 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON TO THE NMR STRUCTURE OF THE B1 DOMAIN

1pgx, resolution 1.66Å

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