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New page: left|200px<br /><applet load="1pjf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pjf" /> '''Solid State NMR structure of the Pf1 Major C...
 
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[[Image:1pjf.jpg|left|200px]]<br /><applet load="1pjf" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1pjf" />
'''Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage'''<br />


==Overview==
==Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage==
The atomic resolution structure of Pf1 coat protein determined by, solid-state NMR spectroscopy of magnetically aligned filamentous, bacteriophage particles in solution is compared to the structures, previously determined by X-ray fiber and neutron diffraction, the, structure of its membrane-bound form, and the structure of fd coat, protein. These structural comparisons provide insights into several, biological properties, differences between class I and class II, filamentous bacteriophages, and the assembly process. The six N-terminal, amino acid residues adopt an unusual "double hook" conformation on the, outside of the bacteriophage particle. The solid-state NMR results, indicate that at 30 degrees C, some of the coat protein subunits assume a, single, fully structured conformation, and some have a few mobile residues, that provide a break between two helical segments, in agreement with, structural models from X-ray fiber and neutron diffraction, respectively., The atomic resolution structure determined by solid-state NMR for residues, 7-14 and 18-46, which excludes the N-terminal double hook and the break, between the helical segments, but encompasses more than 80% of the, backbone including the distinct kink at residue 29, agrees with that, determined by X-ray fiber diffraction with an RMSD value of 2.0 A. The, symmetry and distance constraints determined by X-ray fiber and neutron, diffraction enable the construction of an accurate model of the, bacteriophage particle from the coordinates of the coat protein monomers.
<StructureSection load='1pjf' size='340' side='right'caption='[[1pjf]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1pjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_Pf1 Pseudomonas virus Pf1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjf OCA], [https://pdbe.org/1pjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pjf RCSB], [https://www.ebi.ac.uk/pdbsum/1pjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pjf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAPSD_BPPF1 CAPSD_BPPF1] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).


==About this Structure==
==See Also==
1PJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PJF OCA].
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of the coat protein in Pf1 bacteriophage determined by solid-state NMR spectroscopy., Thiriot DS, Nevzorov AA, Zagyanskiy L, Wu CH, Opella SJ, J Mol Biol. 2004 Aug 13;341(3):869-79. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15288792 15288792]
[[Category: Large Structures]]
[[Category: Pseudomonas phage pf1]]
[[Category: Pseudomonas virus Pf1]]
[[Category: Single protein]]
[[Category: Nevzorov AA]]
[[Category: Nevzorov, A.A.]]
[[Category: Opella SJ]]
[[Category: Opella, S.J.]]
[[Category: Thiriot DS]]
[[Category: Thiriot, D.S.]]
[[Category: Wu CH]]
[[Category: Wu, C.H.]]
[[Category: Zagyanskiy L]]
[[Category: Zagyanskiy, L.]]
[[Category: helical virus]]
[[Category: virus/viral protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:51:47 2007''

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Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage

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