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New page: left|200px<br /><applet load="1plc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1plc, resolution 1.33Å" /> '''ACCURACY AND PRECISI...
 
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[[Image:1plc.gif|left|200px]]<br /><applet load="1plc" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1plc, resolution 1.33&Aring;" />
'''ACCURACY AND PRECISION IN PROTEIN CRYSTAL STRUCTURE ANALYSIS: RESTRAINED LEAST-SQUARES REFINEMENT OF THE CRYSTAL STRUCTURE OF POPLAR PLASTOCYANIN AT 1.33 ANGSTROMS RESOLUTION'''<br />


==Overview==
==ACCURACY AND PRECISION IN PROTEIN CRYSTAL STRUCTURE ANALYSIS: RESTRAINED LEAST-SQUARES REFINEMENT OF THE CRYSTAL STRUCTURE OF POPLAR PLASTOCYANIN AT 1.33 ANGSTROMS RESOLUTION==
The structure of the electron-transfer protein, plastocyanin (99 amino, acids, one Cu atom, 10,500 Da) from poplar leaves, has been refined at, 1.33 A resolution to a residual R = 0.15. The space group is orthorhombic, P2(1)2(1)2(1), a = 29.60 (1), b = 46.86 (3), c = 57.60 (3) A. The 14,303, reflections used in the refinement were obtained from a data set recorded, on a four-circle diffractometer with radiation from a sealed fine-focus, tube, combined with a data set measured on oscillation films exposed at, the DESY synchrotron. The final model comprises 1442 (738 non-H) protein, atoms, one Cu atom and 110 solvent molecules. Nine residues are described, as disordered. The root-mean-square deviation from ideal bond lengths is, 0.016 A and the root-mean-square difference between the positions of the C, alpha atoms in this refined model and in the structure previously refined, at 1.6 A resolution is 0.11 A. The effects of manual model adjustment, resolution, choice of standard values for geometrical parameters, inclusion of H atoms and inclusion of anomalous-scattering corrections on, the copper-site geometry have been explored. The final values of the, Cu-ligand bond lengths are: Cu--N(His37) 1.91, Cu--S(Cys84) 2.07, Cu--N(His87) 2.06, Cu--S(Met92) 2.82 A.
<StructureSection load='1plc' size='340' side='right'caption='[[1plc]], [[Resolution|resolution]] 1.33&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1plc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1pcy 1pcy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PLC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.33&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1plc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1plc OCA], [https://pdbe.org/1plc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1plc RCSB], [https://www.ebi.ac.uk/pdbsum/1plc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1plc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PLAS1_POPNI PLAS1_POPNI] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pl/1plc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1plc ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1PLC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 1PCY. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PLC OCA].
*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Accuracy and precision in protein structure analysis: restrained least-squares refinement of the structure of poplar plastocyanin at 1.33 A resolution., Guss JM, Bartunik HD, Freeman HC, Acta Crystallogr B. 1992 Dec 1;48 ( Pt 6):790-811. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1492962 1492962]
[[Category: Large Structures]]
[[Category: Populus nigra]]
[[Category: Populus nigra]]
[[Category: Single protein]]
[[Category: Freeman HC]]
[[Category: Freeman, H.C.]]
[[Category: Guss JM]]
[[Category: Guss, J.M.]]
[[Category: CU]]
[[Category: electron transport]]
 
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Retrieved from "https://proteopedia.org/1plc"