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New page: left|200px<br /><applet load="1plq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1plq, resolution 2.3Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1plq.gif|left|200px]]<br /><applet load="1plq" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1plq, resolution 2.3&Aring;" />
'''CRYSTAL STRUCTURE OF THE EUKARYOTIC DNA POLYMERASE PROCESSIVITY FACTOR PCNA'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE EUKARYOTIC DNA POLYMERASE PROCESSIVITY FACTOR PCNA==
The crystal structure of the processivity factor required by eukaryotic, DNA polymerase delta, proliferating cell nuclear antigen (PCNA) from S., cerevisiae, has been determined at 2.3 A resolution. Three PCNA molecules, each containing two topologically identical domains, are tightly, associated to form a closed ring. The dimensions and electrostatic, properties of the ring suggest that PCNA encircles duplex DNA, providing a, DNA-bound platform for the attachment of the polymerase. The trimeric PCNA, ring is strikingly similar to the dimeric ring formed by the beta subunit, (processivity factor) of E. coli DNA polymerase III holoenzyme, with which, it shares no significant sequence identity. This structural correspondence, further substantiates the mechanistic connection between eukaryotic and, prokaryotic DNA replication that has been suggested on biochemical, grounds.
<StructureSection load='1plq' size='340' side='right'caption='[[1plq]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1plq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PLQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PLQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1plq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1plq OCA], [https://pdbe.org/1plq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1plq RCSB], [https://www.ebi.ac.uk/pdbsum/1plq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1plq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PCNA_YEAST PCNA_YEAST] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.<ref>PMID:11545742</ref> <ref>PMID:12226657</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pl/1plq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1plq ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1PLQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PLQ OCA].
*[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA., Krishna TS, Kong XP, Gary S, Burgers PM, Kuriyan J, Cell. 1994 Dec 30;79(7):1233-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8001157 8001157]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Burgers PM]]
[[Category: Burgers, P.M.]]
[[Category: Gary S]]
[[Category: Gary, S.]]
[[Category: Kong X-P]]
[[Category: Kong, X.P.]]
[[Category: Krishna TSR]]
[[Category: Krishna, T.S.R.]]
[[Category: Kuriyan J]]
[[Category: Kuriyan, J.]]
[[Category: HG]]
[[Category: dna-binding]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:54:43 2007''

Latest revision as of 08:09, 14 February 2024

CRYSTAL STRUCTURE OF THE EUKARYOTIC DNA POLYMERASE PROCESSIVITY FACTOR PCNA

1plq, resolution 2.30Å

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