1pvz: Difference between revisions
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New page: left|200px<br /><applet load="1pvz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pvz" /> '''Solution Structure of BmP07, A Novel Potassi... |
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== | ==Solution Structure of BmP07, A Novel Potassium Channel Blocker from Scorpion Buthus martensi Karsch, 15 structures== | ||
A natural K+ channel blocker, BmKK2 (a member of scorpion toxin subfamily | <StructureSection load='1pvz' size='340' side='right'caption='[[1pvz]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1pvz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PVZ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pvz OCA], [https://pdbe.org/1pvz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pvz RCSB], [https://www.ebi.ac.uk/pdbsum/1pvz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pvz ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/KA142_MESMA KA142_MESMA] Inhibits potassium channels. May be active towards small conductance calcium-activated potassium channels (KCNN, SK), and less active towards voltage-gated potassium channels (Kv/KCN).<ref>PMID:15146482</ref> <ref>PMID:15208022</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A natural K+ channel blocker, BmKK2 (a member of scorpion toxin subfamily alpha-KTx 14), which is composed of 31 amino acid residues and purified from the venom of the Chinese scorpion Buthus martensi Karsch, was characterized using whole-cell patch-clamp recording in rat hippocampal neurons. The three dimensional structure of BmKK2 was determined with two-dimensional NMR spectroscopy and molecular modelling techniques. In solution this toxin adopted a common alpha/beta-motif, but showed distinct local conformation in the loop between alpha-helix and beta-sheet in comparison with typical short-chain scorpion toxins (e.g., CTX and NTX). Also, the alpha helix is shorter and the beta-sheet element is smaller (each strand consisted only two residues). The unusual structural feature of BmKK2 was attributed to the shorter loop between the alpha-helix and beta-sheet and the presence of two consecutive Pro residues at position 21 and 22 in the loop. Moreover, two models of BmKK2/hKv1.3 channel and BmKK2/rSK2 channel complexes were simulated with docking calculations. The results demonstrated the existence of a alpha-mode binding between the toxin and the channels. The model of BmKK2/rSK2 channel complex exhibited favorable contacts both in electrostatic and hydrophobic, including a network of five hydrogen bonds and bigger interface containing seven pairs of inter-residue interactions. In contrast, the model of BmKK2/hKv1.3 channel complex, containing only three pairs of inter-residue interactions, exhibited poor contacts and smaller interface. The results well explained its lower activity towards Kv channel, and predicted that it may prefer a type of SK channel with a narrower entryway as its specific receptor. | |||
Solution structure of BmKK2, a new potassium channel blocker from the venom of chinese scorpion Buthus martensi Karsch.,Zhang N, Li M, Chen X, Wang Y, Wu G, Hu G, Wu H Proteins. 2004 Jun 1;55(4):835-45. PMID:15146482<ref>PMID:15146482</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1pvz" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mesobuthus martensii]] | [[Category: Mesobuthus martensii]] | ||
[[Category: Hu G]] | |||
[[Category: Hu | [[Category: Li M]] | ||
[[Category: Li | [[Category: Ou L]] | ||
[[Category: Ou | [[Category: Wang Y]] | ||
[[Category: Wang | [[Category: Wu H]] | ||
[[Category: Wu | [[Category: Zhang N]] | ||
[[Category: Zhang | [[Category: Zhang Q]] | ||
[[Category: Zhang | |||
