1odz: Difference between revisions

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~~[[Image:1odz.gif|left|200px]]<br />~~

~~<applet load="1odz" size="450" color="white" frame="true" align="right" spinBox="true"~~

~~caption="1odz, resolution 1.40Å" />~~

~~'''EXPANSION OF THE GLYCOSYNTHASE REPERTOIRE TO PRODUCE DEFINED MANNO-OLIGOSACCHARIDES'''<br />~~

==~~Overview~~==

==Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides==

Mutant endo-mannanases, in which the catalytic nucleophile has been, replaced, function as glycosynthases in the synthesis of, manno-oligosaccharides of defined lengths.

== Structural highlights ==

<table><tr><td colspan='2'>[[1odz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ODZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ODZ FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1odz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1odz OCA], [https://pdbe.org/1odz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1odz RCSB], [https://www.ebi.ac.uk/pdbsum/1odz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1odz ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/MANA_CELJU MANA_CELJU] Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.<ref>PMID:7848261</ref> <ref>PMID:11064195</ref>

== Evolutionary Conservation ==

[[Image:Consurf_key_small.gif|200px|right]]

Check<jmol>

<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/od/1odz_consurf.spt"</scriptWhenChecked>

<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>

<text>to colour the structure by Evolutionary Conservation</text>

</jmolCheckbox>

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1odz ConSurf].

== Publication Abstract from PubMed ==

Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined lengths.

~~==About this Structure==~~

Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides.,Jahn M, Stoll D, Warren RA, Szabo L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG Chem Commun (Camb). 2003 Jun 21;(12):1327-9. PMID:12841226<ref>PMID:12841226</ref>

~~1ODZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure~~ of ~~sequence from [[http://en~~.~~wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]] with ZN~~, ~~NA and TRS as [[http://en.wikipedia~~.~~org/wiki/ligands ligands]]. Active as [[http~~:~~//en.wikipedia~~.~~org/wiki/ ]], with EC number [[http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78]]. Full crystallographic information is available from [[http~~:/~~/ispc.weizmann.ac.il/oca-bin/ocashort?id=1ODZ OCA]].~~

~~==Reference==~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~Expansion~~ of the ~~glycosynthase repertoire to produce defined manno-oligosaccharides~~.~~, Jahn M, Stoll D, Warren RA, Szabo L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG, Chem Commun (Camb)~~. ~~2003 Jun 21;(12):1327~~-~~9. PMID~~:[~~http:~~//~~ispc.weizmann.ac.il//pmbin/getpm?pmid=12841226 12841226]~~

</div>

==See Also==

*[[Mannosidase 3D structures|Mannosidase 3D structures]]

== References ==

__TOC__

</StructureSection>

[[Category: Cellvibrio japonicus]]

[[Category: ~~Single protein~~]]

[[Category: Large Structures]]

[[Category: Davies~~, G.J.~~]]

[[Category: Davies GJ]]

[[Category: Ducros~~, V.M.A.~~]]

[[Category: Ducros VMA]]

[[Category: Gilbert~~, H.J.~~]]

[[Category: Gilbert HJ]]

[[Category: Jahn, M.]]

[[Category: Jahn M]]

[[Category: Singh, P.]]

[[Category: Singh P]]

[[Category: Stoll, D.]]

[[Category: Stoll D]]

[[Category: Szabo, L.]]

[[Category: Szabo L]]

[[Category: Warren~~, R.A.J.~~]]

[[Category: Warren RAJ]]

[[Category: Withers~~, S.G.]]~~

[[Category: Withers SG]]

~~[[Category: NA]]~~

~~[[Category: TRS]]~~

~~[[Category: ZN]]~~

~~[[Category: family 26]]~~

~~[[Category: glycoside-hydrolase]]~~

~~[[Category: glycosynthase]]~~

~~[[Category: mannanase]]~~

~~[[Category: manno-oligosaccharide]~~]

~~''Page seeded by [http://ispc.weizmann.ac.il/oca OCA~~ ] ~~on Mon Oct 29 20:49:47 2007''~~

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@@ Line 1: / Line 1: @@
-[[Image:1odz.gif|left|200px]]<br />
-<applet load="1odz" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1odz, resolution 1.40&Aring;" />
-'''EXPANSION OF THE GLYCOSYNTHASE REPERTOIRE TO PRODUCE DEFINED MANNO-OLIGOSACCHARIDES'''<br />
-==Overview==
+==Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides==
-Mutant endo-mannanases, in which the catalytic nucleophile has been, replaced, function as glycosynthases in the synthesis of, manno-oligosaccharides of defined lengths.
+<StructureSection load='1odz' size='340' side='right'caption='[[1odz]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1odz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ODZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ODZ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1odz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1odz OCA], [https://pdbe.org/1odz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1odz RCSB], [https://www.ebi.ac.uk/pdbsum/1odz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1odz ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MANA_CELJU MANA_CELJU] Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.<ref>PMID:7848261</ref> <ref>PMID:11064195</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/od/1odz_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1odz ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined lengths.
-==About this Structure==
+Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides.,Jahn M, Stoll D, Warren RA, Szabo L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG Chem Commun (Camb). 2003 Jun 21;(12):1327-9. PMID:12841226<ref>PMID:12841226</ref>
-ODZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]] with ZN, NA and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ODZ OCA]].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides., Jahn M, Stoll D, Warren RA, Szabo L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG, Chem Commun (Camb). 2003 Jun 21;(12):1327-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12841226 12841226]
+</div>
+<div class="pdbe-citations 1odz" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Mannosidase 3D structures|Mannosidase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Cellvibrio japonicus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Davies, G.J.]]
+[[Category: Davies GJ]]
-[[Category: Ducros, V.M.A.]]
+[[Category: Ducros VMA]]
-[[Category: Gilbert, H.J.]]
+[[Category: Gilbert HJ]]
-[[Category: Jahn, M.]]
+[[Category: Jahn M]]
-[[Category: Singh, P.]]
+[[Category: Singh P]]
-[[Category: Stoll, D.]]
+[[Category: Stoll D]]
-[[Category: Szabo, L.]]
+[[Category: Szabo L]]
-[[Category: Warren, R.A.J.]]
+[[Category: Warren RAJ]]
-[[Category: Withers, S.G.]]
+[[Category: Withers SG]]
-[[Category: NA]]
-[[Category: TRS]]
-[[Category: ZN]]
-[[Category: family 26]]
-[[Category: glycoside-hydrolase]]
-[[Category: glycosynthase]]
-[[Category: mannanase]]
-[[Category: manno-oligosaccharide]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:49:47 2007''