2b7c: Difference between revisions

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{{Seed}}
[[Image:2b7c.png|left|200px]]


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==Yeast guanine nucleotide exchange factor eEF1Balpha K205A mutant in complex with eEF1A==
The line below this paragraph, containing "STRUCTURE_2b7c", creates the "Structure Box" on the page.
<StructureSection load='2b7c' size='340' side='right'caption='[[2b7c]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2b7c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B7C FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b7c OCA], [https://pdbe.org/2b7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b7c RCSB], [https://www.ebi.ac.uk/pdbsum/2b7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b7c ProSAT]</span></td></tr>
{{STRUCTURE_2b7c|  PDB=2b7c  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/EF1A_YEAST EF1A_YEAST]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/2b7c_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b7c ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To sustain efficient translation, eukaryotic elongation factor B alpha (eEF1B alpha) functions as the guanine nucleotide exchange factor for eEF1A. Stopped-flow kinetics using 2'-(or 3')-O-N-methylanthraniloyl (mant)-GDP showed spontaneous release of nucleotide from eEF1A is extremely slow and accelerated 700-fold by eEF1B alpha. The eEF1B alpha-stimulated reaction was inhibited by Mg2+ with a K(1/2) of 3.8 mM. Previous structural studies predicted the Lys-205 residue of eEF1B alpha plays an important role in promoting nucleotide exchange by disrupting the Mg2+ binding site. Co-crystal structures of the lethal K205A mutant in the catalytic C terminus of eEF1B alpha with eEF1A and eEF1A.GDP established that the lethality was not due to a structural defect. Instead, the K205A mutant drastically reduced the nucleotide exchange activity even at very low concentrations of Mg2+. A K205R eEF1B alpha mutant on the other hand was functional in vivo and showed nearly wild-type nucleotide dissociation rates but almost no sensitivity to Mg2+. These results indicate the significant role of Mg2+ in the nucleotide exchange reaction by eEF1B alpha and establish the catalytic function of Lys-205 in displacing Mg2+ from its binding site.


===Yeast guanine nucleotide exchange factor eEF1Balpha K205A mutant in complex with eEF1A===
Mg2+ and a key lysine modulate exchange activity of eukaryotic translation elongation factor 1B alpha.,Pittman YR, Valente L, Jeppesen MG, Andersen GR, Patel S, Kinzy TG J Biol Chem. 2006 Jul 14;281(28):19457-68. Epub 2006 May 4. PMID:16675455<ref>PMID:16675455</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2b7c" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_16675455}}, adds the Publication Abstract to the page
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 16675455 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_16675455}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2B7C is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7C OCA].
 
==Reference==
Mg2+ and a key lysine modulate exchange activity of eukaryotic translation elongation factor 1B alpha., Pittman YR, Valente L, Jeppesen MG, Andersen GR, Patel S, Kinzy TG, J Biol Chem. 2006 Jul 14;281(28):19457-68. Epub 2006 May 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16675455 16675455]
 
Structural basis for nucleotide exchange and competition with tRNA in the yeast elongation factor complex eEF1A:eEF1Balpha., Andersen GR, Pedersen L, Valente L, Chatterjee I, Kinzy TG, Kjeldgaard M, Nyborg J, Mol Cell. 2000 Nov;6(5):1261-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11106763 11106763]
 
Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex., Andersen GR, Valente L, Pedersen L, Kinzy TG, Nyborg J, Nat Struct Biol. 2001 Jun;8(6):531-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11373622 11373622]
[[Category: Protein complex]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Andersen, G R.]]
[[Category: Andersen GR]]
[[Category: Jeppesen, M G.]]
[[Category: Jeppesen MG]]
[[Category: Kinzy, T G.]]
[[Category: Kinzy TG]]
[[Category: Patel, S.]]
[[Category: Patel S]]
[[Category: Pittman, Y R.]]
[[Category: Pittman YR]]
[[Category: Valente, L.]]
[[Category: Valente L]]
[[Category: Eef1a]]
[[Category: Eef1balpha]]
[[Category: G-protein/gef complex]]
 
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Retrieved from "https://proteopedia.org/2b7c"