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New page: left|200px<br /><applet load="1q5z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q5z, resolution 1.8Å" /> '''Crystal Structure of ...
 
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[[Image:1q5z.jpg|left|200px]]<br /><applet load="1q5z" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1q5z, resolution 1.8&Aring;" />
'''Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)'''<br />


==Overview==
==Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)==
Like many bacterial pathogens, Salmonella spp. use a type III secretion, system to inject virulence proteins into host cells. The Salmonella, invasion protein A (SipA) binds host actin, enhances its polymerization, near adherent extracellular bacteria, and contributes to cytoskeletal, rearrangements that internalize the pathogen. By combining x-ray, crystallography of SipA with electron microscopy and image analysis of, SipA-actin filaments, we show that SipA functions as a "molecular staple,", in which a globular domain and two nonglobular "arms" mechanically, stabilize the filament by tethering actin subunits in opposing strands., Deletion analysis of the tethering arms provides strong support for this, model.
<StructureSection load='1q5z' size='340' side='right'caption='[[1q5z]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1q5z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q5Z FirstGlance]. <br>
1Q5Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q5z OCA], [https://pdbe.org/1q5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q5z RCSB], [https://www.ebi.ac.uk/pdbsum/1q5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q5z ProSAT]</span></td></tr>
==Reference==
</table>
Salmonella SipA polymerizes actin by stapling filaments with nonglobular protein arms., Lilic M, Galkin VE, Orlova A, VanLoock MS, Egelman EH, Stebbins CE, Science. 2003 Sep 26;301(5641):1918-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14512630 14512630]
== Function ==
[[Category: Salmonella typhimurium]]
[https://www.uniprot.org/uniprot/SIPA_SALTY SIPA_SALTY] Actin-binding protein that interferes with host cell actin cytoskeleton. It stimulates actin polymerization and counteracts F-actin destabilizing proteins. Potentiates SipC activity; both are required for an efficient bacterial internalization. In vitro, forms a complex with host cell protein T-plastin increasing actin bundling. It inhibits ADF/cofilin-directed depolymerization both by preventing binding of ADF and cofilin and by displacing them from F-actin. Also protects F-actin from gelsolin-directed severing and reanneals gelsolin-severed F-actin fragments.<ref>PMID:10092234</ref> <ref>PMID:14992720</ref>
[[Category: Single protein]]
== References ==
[[Category: Egelman, E.H.]]
<references/>
[[Category: Galkin, V.E.]]
__TOC__
[[Category: Lilic, M.]]
</StructureSection>
[[Category: Orlova, A.]]
[[Category: Large Structures]]
[[Category: Stebbins, C.E.]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: VanLoock, M.S.]]
[[Category: Egelman EH]]
[[Category: cell invasion]]
[[Category: Galkin VE]]
 
[[Category: Lilic M]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:24:43 2007''
[[Category: Orlova A]]
[[Category: Stebbins CE]]
[[Category: VanLoock MS]]

Latest revision as of 08:13, 14 February 2024

Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)

1q5z, resolution 1.80Å

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