2jz1: Difference between revisions

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{{Seed}}
[[Image:2jz1.png|left|200px]]


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==DSX_long==
The line below this paragraph, containing "STRUCTURE_2jz1", creates the "Structure Box" on the page.
<StructureSection load='2jz1' size='340' side='right'caption='[[2jz1]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2jz1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JZ1 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jz1 OCA], [https://pdbe.org/2jz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jz1 RCSB], [https://www.ebi.ac.uk/pdbsum/2jz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jz1 ProSAT]</span></td></tr>
{{STRUCTURE_2jz1|  PDB=2jz1  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/DSX_DROME DSX_DROME] Controls somatic sexual differentiation. Binds directly and specifically to the FBE (fat body enhancer) of the yolk protein 1 and 2 genes (Yp1 and Yp2). This enhancer is sufficient to direct the female-specific transcription characteristic of the Yp genes in adult fat bodies. Involved in regulation of male-specific expression of takeout in brain-associated fat body.<ref>PMID:12435630</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jz/2jz1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jz1 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The DSX (Doublesex) transcription factor regulates somatic sexual differentiation in Drosophila. Female and male isoforms (DSX F and DSX M) are formed due to sex-specific RNA splicing. DNA recognition, mediated by a shared N-terminal zinc module (the DM domain), is enhanced by a C-terminal dimerization element. Sex-specific extension of this element in DSX F and DSX M leads to assembly of distinct transcriptional preinitiation complexes. Here, we describe the structure of the extended C-terminal dimerization domain of DSX F as determined by multidimensional NMR spectroscopy. The core dimerization element is well ordered, giving rise to a dense network of interresidue nuclear Overhauser enhancements. The structure contains dimer-related UBA folds similar to those defined by x-ray crystallographic studies of a truncated domain. Whereas the proximal portion of the female tail extends helix 3 of the UBA fold, the distal tail is disordered. Ala substitutions in the proximal tail disrupt the sex-specific binding of IX (Intersex), an obligatory partner protein and putative transcriptional coactivator; IX-DSX F interaction is, by contrast, not disrupted by truncation of the distal tail. Mutagenesis of the UBA-like dimer of DSX F highlights the importance of steric and electrostatic complementarity across the interface. Two temperature-sensitive mutations at this interface have been characterized in yeast model systems. One weakens a network of solvated salt bridges, whereas the other perturbs the underlying nonpolar interface. These mutations confer graded gene-regulatory activity in yeast within a physiological temperature range and so may provide novel probes for genetic analysis of a sex-specific transcriptional program in Drosophila development.


===DSX_long===
Doublesex and the regulation of sexual dimorphism in Drosophila melanogaster: structure, function, and mutagenesis of a female-specific domain.,Yang Y, Zhang W, Bayrer JR, Weiss MA J Biol Chem. 2008 Mar 14;283(11):7280-92. Epub 2008 Jan 9. PMID:18184648<ref>PMID:18184648</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<!--
</div>
The line below this paragraph, {{ABSTRACT_PUBMED_18184648}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2jz1" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 18184648 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_18184648}}
__TOC__
 
</StructureSection>
==About this Structure==
2JZ1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JZ1 OCA].
 
==Reference==
Doublesex and the Regulation of Sexual Dimorphism in Drosophila melanogaster: STRUCTURE, FUNCTION, AND MUTAGENESIS OF A FEMALE-SPECIFIC DOMAIN., Yang Y, Zhang W, Bayrer JR, Weiss MA, J Biol Chem. 2008 Mar 14;283(11):7280-92. Epub 2008 Jan 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18184648 18184648]
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bayrer, J R.]]
[[Category: Bayrer JR]]
[[Category: Weiss, M A.]]
[[Category: Weiss MA]]
[[Category: Yang, Y.]]
[[Category: Yang Y]]
[[Category: Zhang, W.]]
[[Category: Zhang W]]
[[Category: Development]]
[[Category: Double sex]]
[[Category: Gene regulation]]
[[Category: Sex determination]]
[[Category: Transcription]]
[[Category: Ubiquitin]]
 
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Retrieved from "https://proteopedia.org/2jz1"