2a8f: Difference between revisions

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{{Seed}}
[[Image:2a8f.png|left|200px]]


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==beta-cinnamomin after sterol removal==
The line below this paragraph, containing "STRUCTURE_2a8f", creates the "Structure Box" on the page.
<StructureSection load='2a8f' size='340' side='right'caption='[[2a8f]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2a8f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phytophthora_cinnamomi Phytophthora cinnamomi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8F FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8f OCA], [https://pdbe.org/2a8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8f RCSB], [https://www.ebi.ac.uk/pdbsum/2a8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8f ProSAT]</span></td></tr>
{{STRUCTURE_2a8f|  PDB=2a8f  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/ELIB_PHYCI ELIB_PHYCI] Induces local and distal defense responses (incompatible hypersensitive reaction) in plants from the solanaceae and cruciferae families. Elicits leaf necrosis and causes the accumulation of pathogenesis-related proteins. Might interact with the lipidic molecules of the plasma membrane. Elicitins are able to load, carry, and transfer sterols between membranes.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8f_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8f ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was determined in complex with ergosterol at 1.1 A resolution. beta-CIN/ergosterol complex crystallized in the monoclinic space group P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand extraction with chloroform followed by crystallographic analysis yielded a 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The necrotic effect of beta-CIN on tobacco was also shown to remain unchanged. Theoretical docking studies of the triterpene lupeol to beta-CIN provided an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.


===beta-cinnamomin after sterol removal===
Crystal structures of the free and sterol-bound forms of beta-cinnamomin.,Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:16249127<ref>PMID:16249127</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_16249127}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2a8f" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 16249127 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_16249127}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2A8F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phytophthora_cinnamomi Phytophthora cinnamomi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8F OCA].
 
==Reference==
Crystal structures of the free and sterol-bound forms of beta-cinnamomin., Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA, Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16249127 16249127]
 
Structure of beta-cinnamomin, a protein toxic to some plant species., Rodrigues ML, Archer M, Martel P, Jacquet A, Cravador A, Carrondo MA, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1314-21. Epub 2002, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12136143 12136143]
 
Identification of an elicitin gene cluster in Phytophthora cinnamomi., Duclos J, Fauconnier A, Coelho AC, Bollen A, Cravador A, Godfroid E, DNA Seq. 1998;9(4):231-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10520754 10520754]
[[Category: Phytophthora cinnamomi]]
[[Category: Phytophthora cinnamomi]]
[[Category: Single protein]]
[[Category: Archer M]]
[[Category: Archer, M.]]
[[Category: Baptista RP]]
[[Category: Baptista, R P.]]
[[Category: Carrondo MA]]
[[Category: Carrondo, M A.]]
[[Category: Cravador A]]
[[Category: Cravador, A.]]
[[Category: Enguita FJ]]
[[Category: Enguita, F J.]]
[[Category: Martel P]]
[[Category: Martel, P.]]
[[Category: Melo EP]]
[[Category: Melo, E P.]]
[[Category: Miranda S]]
[[Category: Miranda, S.]]
[[Category: Rodrigues ML]]
[[Category: Rodrigues, M L.]]
[[Category: Sousa N]]
[[Category: Sousa, N.]]
[[Category: Thomaz M]]
[[Category: Thomaz, M.]]
[[Category: Elicitin]]
[[Category: Phytopathogen]]
[[Category: Phytophthora]]
[[Category: Sterol carrier protein]]
 
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Retrieved from "https://proteopedia.org/2a8f"